An electron microscopic study on the innervation in the taste buds of the mouse circumvallate papillae.
An electron microscopic study on the innervation in the taste buds of the mouse circumvallate papillae.
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小鼠周围乳头味蕾神经支配的电子显微镜研究。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
M. Takeda
中科院分区：
文献类型：
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作者：
M. Takeda
用电镜观察了小鼠轮廓乳头味蕾的肾上腺素能神经支配。典型的传入突触接触，与邻近的神经末梢的细胞质中的突触囊泡的膜和聚集体的密度增加，被证明之间的III型细胞和神经末梢。沿着Ⅱ型细胞与神经末梢的接触区，细胞膜下常有内质网池，神经末梢含有较多突触大小的小泡。这种神经支配似乎是传出性的。为了检测肾上腺素能神经的供应，在用L-DOPA和尼阿丁胺预处理后注射5-羟基多巴胺（5-OH-DA）。老鼠的味蕾下面的结缔组织中有大量的肾上腺素能神经纤维。在极少数情况下，肾上腺素能神经穿透味蕾的基底层并与芽细胞接触。味蕾周围的上皮细胞中分布有肾上腺素能神经。在5-OH-DA标记的肾上腺素能神经纤维周围发现乙酰胆碱酯酶活性的反应产物。
Taste buds of the mouse circumvallate papillae were studied by electron microscopy to elucidate the innervation involving the adrenergic nerve supply. Typical afferent synaptic contacts, with increased density of the membranes and aggregations of synaptic vesicles in the cytoplasm adjacent to the nerve endings, were demonstrated between the type III cells and the nerve endings. Along the regions of contact between the type II cells and the nerve endings, cisternae of endoplasmic reticulum were often seen beneath the cell membrane, and the nerve endings contained relatively many synaptic-sized vesicles. Such an innervation seems to be efferent in nature. For the detection of the adrenergic nerve supply, 5-hydroxydopamine (5-OH-DA) was injected after pretreatment with L-DOPA and nialamide. The mice showed numerous adrenergic nerve fibers in the connective tissue underlying the taste buds. On very few occasions, the adrenergic nerves penetrated the basal lamina of the taste buds and came into contact with the bud cells. Some adrenergic nerves were distributed among the epithelial cells around the taste buds. The reaction product from acetylcholine esterase activity was found around the adrenergic nerve fibers labeled with 5-OH-DA.