Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus.
Genome sequence and virulence variation-related transcriptome profiles of Curvularia lunata, an important maize pathogenic fungus.
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玉米重要病原真菌新月弯孢菌的基因组序列和毒力变异相关转录组谱
DOI：
10.1186/1471-2164-15-627
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发表时间：
2014-07-24
期刊：
影响因子：
4.4
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Gao S;Li Y;Gao J;Suo Y;Fu K;Li Y;Chen J
研究背景：新月弯孢菌（Curvularia lunata）是玉米叶部重要的病原真菌，广泛分布于我国玉米产区。该病原菌的基因组测序结果为全面了解其毒力机制提供了重要信息。lunata菌株系统基因组分析表明，C. lunata是由玉米小斑病菌（Copolaris maydis，Cochliobolus heterostrophus）进化而来。基于转座酶和重复诱导的点突变的分析，高毒力菌株具有进化成其他致病菌株的高潜力。C. lunata具有较小比例的分泌蛋白以及B。比昆虫病原真菌多。C. lunata和B.玉米的蛋白质编码基因与病原体-宿主相互作用数据库中实验证明的病原体基因高度同源的比例相似。但是，相对于B。maydis、玉米C. lunata不仅拥有许多扩展的蛋白质家族，包括MFS转运蛋白、G蛋白偶联受体、用于转运、信号转导或降解的蛋白激酶和蛋白酶，而且还拥有许多收缩家族，包括细胞色素P450、脂肪酶、糖苷水解酶和聚酮合酶，用于解毒、水解或次生代谢产物的合成，预计这些对于真菌在不同逆境环境中的生存至关重要。对一株弱毒力C.结果表明，在逆境条件下，该菌的毒力增强与毒素和黑色素生物合成途径有关，且这两条途径可能存在一定的重叠。lunata。
Background:Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism.Results:We report the genome sequences of a highly virulent C. lunata strain. Phylogenomic analysis indicates that C. lunata was evolved from Bipolaris maydis (Cochliobolus heterostrophus). The highly virulent strain has a high potential to evolve into other pathogenic stains based on analyses on transposases and repeat-induced point mutations. C. lunata has a smaller proportion of secreted proteins as well as B. maydis than entomopathogenic fungi. C. lunata and B. maydis have a similar proportion of protein-encoding genes highly homologous to experimentally proven pathogenic genes from pathogen-host interaction database. However, relative to B. maydis, C. lunata possesses not only many expanded protein families including MFS transporters, G-protein coupled receptors, protein kinases and proteases for transport, signal transduction or degradation, but also many contracted families including cytochrome P450, lipases, glycoside hydrolases and polyketide synthases for detoxification, hydrolysis or secondary metabolites biosynthesis, which are expected to be crucial for the fungal survival in varied stress environments. Comparative transcriptome analysis between a lowly virulent C. lunata strain and its virulence-increased variant induced by resistant host selection reveals that the virulence increase of the pathogen is related to pathways of toxin and melanin biosynthesis in stress environments, and that the two pathways probably have some overlaps.Conclusions:The data will facilitate a full revelation of pathogenic mechanism and a better understanding of virulence differentiation of C. lunata.