A beginner's guide to rigor and reproducibility in fluorescence imaging experiments.
A beginner's guide to rigor and reproducibility in fluorescence imaging experiments.
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DOI：
10.1091/mbc.e17-05-0276
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发表时间：
2018-07-01
影响因子：
3.3
通讯作者：
Kitaoka M
中科院分区：
文献类型：
--
作者：
Lee JY;Kitaoka M
荧光显微镜是研究细胞生物学机制不可或缺的手段。随着遗传编码荧光蛋白和超分辨率方法等尖端工具的发展，光学显微镜在提供从细菌裂变到癌症转移等广泛现象的洞察方面比以往任何时候都更加强大。然而，与所有实验方法一样，必须注意确保可靠和可重复的数据收集，分析和报告。每个成像实验的每一步，从设计到执行到通信到数据管理，都应该严格评估偏倚，严谨性和再现性。本《透视》为设计和执行荧光成像实验提供了基本的“最佳实践”指南，目的是向研究人员介绍有助于他们精确获取图像的概念。
Fluorescence light microscopy is an indispensable approach for the investigation of cell biological mechanisms. With the development of cutting-edge tools such as genetically encoded fluorescent proteins and superresolution methods, light microscopy is more powerful than ever at providing insight into a broad range of phenomena, from bacterial fission to cancer metastasis. However, as with all experimental approaches, care must be taken to ensure reliable and reproducible data collection, analysis, and reporting. Each step of every imaging experiment, from design to execution to communication to data management, should be critically assessed for bias, rigor, and reproducibility. This Perspective provides a basic “best practices” guide for designing and executing fluorescence imaging experiments, with the goal of introducing researchers to concepts that will help empower them to acquire images with rigor.