A concentration dependent study of acoustic plate mode immunosensor response using antigen/antibody systems with different binding ability
A concentration dependent study of acoustic plate mode immunosensor response using antigen/antibody systems with different binding ability
复制标题
DOI：
10.1109/58.726446
复制
发表时间：
1998-09-01
影响因子：
3.6
通讯作者：
Bender, F
中科院分区：
文献类型：
--
作者：
Dahint, R;Bender, F
声板模式传感器已被用于监测作为抗原浓度的函数的免疫化学反应。在这些研究中，抗体通过巯基乙醇、氨基硅烷和戊二醛共价连接到涂金的传感表面。已经使用了两种抗原/抗体模型系统，其相互结合的能力不同。对于在约150 MHz下的传感器操作，在两种情况下获得约0.5 μ g/ml的检测限。结合常数的值在两个系统之间没有显着差异。它们约为1。10（8）l/mole，并且完全落入均相免疫测定所报告的结合常数范围内。两个模型系统获得的传感器响应的比较表明，约70%的固定化抗体是活性的。
Acoustic plate mode sensors have been used to monitor immunochemical reactions as a function of antigen concentration. In the studies, antibodies were covalently linked to the gold-coated sensing surface via mercaptoethanol, aminosilane, and glutaraldehyde. Two antigen/antibody model systems that differ in their ability to mutually bind one another have been used. For sensor operation at about 150 MHz, a detection limit of approximately 0.5 mu g/ml was obtained in both cases. No significant difference between the two systems was found for the value of the binding constants. They amount to about 1 . 10(8) l/mole and fall well into the range of binding constants reported for homogeneous immunoassays. A comparison of the sensor response obtained for the two model systems shows that about 70% of the immobilized antibodies are active.