Coincident activation of Th2 T cells with onset of the disease and differential expression of GRO-gamma in peripheral blood leukocytes in minimal change disease
Coincident activation of Th2 T cells with onset of the disease and differential expression of GRO-gamma in peripheral blood leukocytes in minimal change disease
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DOI：
10.1159/000101371
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发表时间：
2007-01-01
影响因子：
4.2
通讯作者：
Lou, Ya-Huan
中科院分区：
文献类型：
--
作者：
Adrogue, Horacio E.;Borillo, Jason;Lou, Ya-Huan
背景：Th 2 T细胞/NF κ B B参与微小病变性疾病（MCD）已被假定。一个有前途的，但未经证实的肾小球通透性因子（GPF）从MCD T细胞已被描述。我们探讨GPF是否是Th 2细胞活化的结果。方法：对16例MCD患者和7例正常人外周血白细胞（PBL）进行分析，并进行统计学比较。结果如下：流式细胞术显示MCD中CD 4 + T细胞群显著扩增，并且CD 4 + T细胞中的CD 69+细胞显著增加，表明T细胞活化与疾病发作一致。从新鲜分离的PBL或在体外激活后的RNA的RT-PCR显示高水平表达的Th 2细胞因子白细胞介素-4在所有MCD患者。重要的是，血清抗体微阵列分析和PBL mRNA的RT-PCR显示，与7个对照组中的一个相比，所有MCD患者中CXC趋化因子GRO-γ（生长相关癌基因）的表达。结论：我们的研究结果揭示了MCD的发病和Th 2细胞的激活之间的关联。GRO家族与内皮细胞的功能有关，其表达受NF κ B B调节。因此，GRO-γ是MCD中Th 2相关GPF的有希望的候选者。版权所有（C）2007 S. Karger AG，巴塞尔。
Background: Involvement of Th2 T cells/NF kappa B in minimal change disease ( MCD) has been postulated. A promising but unconfirmed glomerular permeability factor ( GPF) from MCD T cells has been described. We explored whether GPF was the consequence of Th2 cell activation. Methods: Peripheral blood leukocytes ( PBL) from 16 MCD patients and 7 normal controls were analyzed and the results were statistically compared. Results: Flow cytometry demonstrated a significant expansion of CD4+ T cell population and dramatically increased CD69+ cells among CD4+ T cells in MCD, suggesting coincident activation of T cells with onset of the disease. RT-PCR on RNA from either freshly isolated PBL or post in vitro activation showed high-level expression of the Th2 cytokine interleukin-4 in all MCD patients. Importantly, both antibody microarray assay on sera and RT-PCR on mRNA of PBL revealed expression of a CXC chemokine GRO-gamma ( growth-related oncogene) in all MCD patients as compared with one of 7 controls. Conclusions: Our results reveal an association between onset of MCD and activation of Th2 cells. The GRO family has been implicated in the function of endothelial cells, and its expression is under NF kappa B regulation. Thus, GRO-gamma is a promising candidate for Th2-associated GPF in MCD. Copyright (C) 2007 S. Karger AG, Basel.