A novel wireless ECG system for prolonged monitoring of multiple zebrafish for heart disease and drug screening studies.
A novel wireless ECG system for prolonged monitoring of multiple zebrafish for heart disease and drug screening studies.
复制标题
DOI：
10.1016/j.bios.2021.113808
复制
发表时间：
2022-02-01
影响因子：
12.6
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
Le T;Zhang J;Nguyen AH;Trigo Torres RS;Vo K;Dutt N;Lee J;Ding Y;Xu X;Lau MPH;Cao H
斑马鱼及其突变系已广泛用于心血管研究。在目前的研究中，新的系统，斑马II，提出了长期的心电图（ECG）采集和分析多条斑马鱼在可控的工作环境。Zebra II由灌注系统、仪器、传感器和内部电子系统组成。首先，Zebra II通过各种实验与基准系统iWORX进行了比较。验证显示在数据质量和药物治疗后ECG变化方面具有可比性。随后在需要实时数据评估的实验中研究了麻醉药物和温度变化对斑马鱼心电图的影响。Zebra II的连续麻醉给药能力使ECG采集时间延长至1小时，而现有系统的采集时间为5分钟。这种新颖的、基于云的自动化分析从四种鱼中获得的数据进一步为组合实验提供了有用的解决方案，并有助于节省大量的时间和精力。该系统显示出强大的ECG采集和分析，用于各种应用，包括钠诱导的窦性停搏中的心律失常、温度诱导的心率变化以及Tg（SCN 5A-D1275 N）突变体和野生型鱼中的药物诱导的心律失常。多通道采集还使得能够在斑马鱼模型上实施随机对照试验。开发的心电图系统充满希望并解决了当前的缺点，以大大加速使用斑马鱼的药物筛选应用和其他心血管研究。
Zebrafish and their mutant lines have been extensively used in cardiovascular studies. In the current study, the novel system, Zebra II, is presented for prolonged electrocardiogram (ECG) acquisition and analysis for multiple zebrafish within controllable working environments. The Zebra II is composed of a perfusion system, apparatuses, sensors, and an in-house electronic system. First, the Zebra II is validated in comparison with a benchmark system, namely iWORX, through various experiments. The validation displayed comparable results in terms of data quality and ECG changes in response to drug treatment. The effects of anesthetic drugs and temperature variation on zebrafish ECG were subsequently investigated in experiments that need real-time data assessment. The Zebra II’s capability of continuous anesthetic administration enabled prolonged ECG acquisition up to 1 h compared to that of 5 min in existing systems. The novel, cloud-based, automated analysis with data obtained from four fish further provided a useful solution for combinatorial experiments and helped save significant time and effort. The system showed robust ECG acquisition and analytics for various applications including arrhythmia in sodium induced sinus arrest, temperature-induced heart rate variation, and drug-induced arrhythmia in Tg(SCN5A-D1275N) mutant and wildtype fish. The multiple channel acquisition also enabled the implementation of randomized controlled trials on zebrafish models. The developed ECG system holds promise and solves current drawbacks in order to greatly accelerate drug screening applications and other cardiovascular studies using zebrafish.
登录
查看更多内容
影响因子：
24
作者：
McNair, William P.;Sinagra, Gianfranco;Taylor, Matthew R. G.;Di Lenarda, Andrea;Ferguson, Debra A.;Salcedo, Ernesto E.;Slavov, Dobromir;Zhu, Xiao;Caldwell, John H.;Mestroni, Luisa
通讯作者：
Mestroni, Luisa
DOI：
10.1016/j.vascn.2013.01.007
发表时间：
2013-03-01
影响因子：
1.9
作者：
Chaudhari, Girish Hari;Chennubhotla, Keerthana Sarma;Kulkarni, Pushkar
通讯作者：
Kulkarni, Pushkar
影响因子：
4.6
作者：
Nagasawa, Sayaka;Saitoh, Hisako;Iwase, Hirotaro
通讯作者：
Iwase, Hirotaro
影响因子：
3.4
作者：
Henry BL;Minassian A;Perry W
通讯作者：
Perry W
DOI：
10.1038/nrcardio.2014.85
发表时间：
2014-10
期刊：
Nature reviews. Cardiology
影响因子：
--
作者：
Liu M;Yang KC;Dudley SC Jr
通讯作者：
Dudley SC Jr