Is meconium from healthy newborns actually sterile?
Is meconium from healthy newborns actually sterile?
复制标题
DOI：
10.1016/j.resmic.2007.12.007
复制
发表时间：
2008-04-01
影响因子：
2.6
通讯作者：
Rodriguez, Juan M.
中科院分区：
文献类型：
--
作者：
Jimenez, Esther;Marin, Maria L.;Rodriguez, Juan M.
在以前的研究中，细菌能够从健康新生儿的脐带血和剖腹产获得的小鼠羊水中分离出来。这表明足月胎儿并非完全无菌，可能存在产前母体向婴儿的肠道细菌外流。因此，对21例健康新生儿胎粪中是否存在此类细菌进行了调查。鉴定的分离株主要属于肠球菌属和葡萄球菌属。随后，一组怀孕的小鼠口服接种遗传标记的E。粪菌菌株先前从健康妇女的母乳中分离。在预产期前一天剖腹产接种的动物胎粪中可分离到标记菌株并进行PCR检测。相反，在未接种对照组的样品中未检出。(C)2008年，Elsevier Masson SAS。All rights reserved.
In a previous study, bacteria were able to be isolated from umbilical cord blood of healthy neonates and from murine amniotic fluid obtained by caesarean section. This suggested that term fetuses are not completely sterile and that a prenatal mother-to-child efflux of commensal bacteria may exist. Therefore, the presence of such bacteria in meconium of 21 healthy neonates was investigated. The identified isolates belonged predominantly to the genuses Enterococcus and Staphylococcus. Later, a group of pregnant mice were orally inoculated with a genetically labelled E. fecium strain previously isolated from breast milk of a healthy woman. The labelled strain could be isolated and PCR-detected from meconium of the inoculated animals obtained by caesarean section one day before the predicted date of labor. In contrast, it could not be detected in samples obtained from a non-inoculated control group. (C) 2008 Elsevier Masson SAS. All rights reserved.