Detection of Epstein-Barr virus in breast carcinoma in Egyptian women
Detection of Epstein-Barr virus in breast carcinoma in Egyptian women
复制标题
DOI：
10.1016/j.clinbiochem.2007.12.017
复制
发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Awad, Nahla Mohammad
中科院分区：
文献类型：
--
作者：
Fawzy, Shereen;Sallam, Maha;Awad, Nahla Mohammad
背景：致癌性EB病毒与乳腺癌的关系仍存在争议。工作目标：评估EB病毒与埃及妇女乳腺癌的相关性，并发现乳腺癌的预后因素与EB病毒检测之间可能存在的关系。乳腺导管组织（n = 32）和小叶组织（n = 8）以及纤维囊性疾病患者的乳腺组织（n = 20）作为对照，通过EBV核抗原筛选EBV的存在。结果：EBNA-1阳性表达率为25%（10/40），EBNA-1仅表达于5%~ 60%的肿瘤上皮细胞。EBNA-I阳性的BC标本中8/10为EBV-DNA阳性。两种技术检测的对照标本均为阴性。7/8例（87.5%）EBV-DNA阳性肿瘤与>3个淋巴结受累有关。结论：EB病毒与埃及女性某些侵袭性BC有关，并可能在其病因中起作用。(c)2007年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Background: The association of oncogenic EBV with breast carcinoma (BC) is still in controversy. Aim of work: Assess the association of EBV with BC in Egyptian women and find possible relationship between prognostic factors of BC and EBV detection.Subjects and methods: Paraffin-embedded sections from 40 female patients with primary invasive BC; ductal (n = 32) and lobular (n = 8) and breast tissues from patients with fibrocystic disease (n = 20) as control were screened for presence of EBV by EBV nuclear antigen-1 (EBNA-1) immunostaining and by PCR for EBV-DNA.Results: 10/40 (25%) of the BC specimens stained positively for EBNA-1; EBNA-1 expression was restricted to a fraction 5%-60% of tumor epithelial cells. EBV-DNA was detected in 8/10 of BC specimens positive for EBNA-I. Control specimens were negative by both techniques. 7/8 (87.5%) of EBV-DNA positive tumors were associated with >3 lymph nodes involvement.Conclusion: EBV is associated with some invasive BC in Egyptian females and may play a role in their etiology. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.