MOUSE OTX2 FUNCTIONS IN THE FORMATION AND PATTERNING OF ROSTRAL HEAD
MOUSE OTX2 FUNCTIONS IN THE FORMATION AND PATTERNING OF ROSTRAL HEAD
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DOI：
10.1101/gad.9.21.2646
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发表时间：
1995-11-01
影响因子：
10.5
通讯作者：
AIZAWA, S
中科院分区：
文献类型：
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作者：
MATSUO, I;KURATANI, S;AIZAWA, S
脊椎动物头部的前部在胚胎发生过程中以节段限制模式表达一组同源盒基因。其中，Otx2表达覆盖整个前脑和中脑，并且最早发生。为了研究其在喙头发育中的作用，将突变引入该位点。纯合突变体没有开发前菱形3的结构，表明Otx 2在喙头的形成中的重要作用。与此相反，杂合子突变体显示颅面畸形指定为耳头畸形，受影响的结构似乎对应于最后和最前面的领域的OTx的表达，其中OTx 1不表达。家庭和杂合突变体表型表明Otx2功能作为一个缺口样基因在喙头Hox代码不存在。Otx 2突变表型的进化意义进行了讨论的脑颅和颌骨的创新。
The anterior part of the vertebrate head expresses a group of homeo box genes in segmentally restricted patterns during embryogenesis. Among these, Otx2 expression covers the entire fore- and midbrains and takes place earliest. To examine its role in development of the rostral head, a mutation was introduced into this locus. The homozygous mutants did not develop structures anterior to rhombomere 3, indicating an essential role of Otx2 in the formation of the rostral head. In contrast, heterozygous mutants displayed craniofacial malformations designated as otocephaly; affected structures appeared to correspond to the most posterior and most anterior domains of Otx expression where Otx1 is not expressed. The home- and heterozygous mutant phenotypes suggest Otx2 functions as a gap-like gene in the rostral head where Hox code is not present. The evolutionary significance of Otx2 mutant phenotypes was discussed for the innovation of the neurocranium and the jaw.