Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging
Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging
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DOI：
10.1006/nimg.2002.1194
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发表时间：
2002-10-01
期刊：
影响因子：
5.7
通讯作者：
Hodges, H
中科院分区：
文献类型：
--
作者：
Modo, M;Cash, D;Hodges, H
通过活体神经成像跟踪脑内干细胞移植的能力无疑将有助于我们理解这些细胞如何在神经移植后介导功能恢复。干细胞移植的发展和完善面临的一个主要挑战是绘制原位迁移的空间分布和速率图。在这里，我们报道了一种通过磁共振成像(MRI)跟踪移植的干细胞在缺血损伤的大鼠海马区的方法。在移植前，干细胞在体外被标记为一种新型的双功能造影剂，即磁共振成像和荧光显微镜都能识别的格拉丹明葡聚糖(GRID)，或者是仅在荧光显微镜下可见的PKH26。在植入后的不同时间点，对脑组织进行组织学和体外磁共振成像。只有在预先标记了GRID的情况下，MRI才能识别移植的干细胞，而荧光显微镜可以检测到使用任何一种标记的移植细胞。MRI检测到的网格标记干细胞的分布与荧光显微镜检测到的一致。这些结果表明，网格增强MRI可以可靠地识别移植的干细胞及其在大脑中的迁移。(C)2002年埃尔塞维尔科学公司(美国)。
The ability to track stem cell transplants in the brain by an vivo neuroimaging will undoubtedly aid our understanding of how these cells mediate functional recovery after neural transplantation. One major challenge for the development and refinement of stem cell transplantation is to map the spatial distribution and rate of migration in situ. Here we report a method for tracking transplanted stem cells in the ischemia-damaged rat hippocampus by magnetic resonance imaging (MRI). Before transplantation, stem cells were labeled in vitro either with a novel bifunctional contrast agent, gadolinium rhodamine dextran (GRID), identifiable by both MRI and fluorescence microscopy, or with PKH26, visible exclusively under fluorescence microscopy. At different time points following engraftment, the brains were evaluated by both histology and ex vivo MR imaging. Transplanted stem cells were identified by MRI only if prelabeled with GRID, whereas fluorescence microscopy detected transplanted cells using either label. The distribution of GRID-labeled stem cells identified by MRI corresponded to those detected using fluorescence microscopy. These results demonstrate that GRID-enhanced MRI can reliably identify transplanted stem cells and their migration in the brain. (C) 2002 Elsevier Science (USA).