The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors
The functional repertoire of prokaryote cellulosomes includes the serpin superfamily of serine proteinase inhibitors
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DOI：
10.1111/j.1365-2958.2006.05182.x
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发表时间：
2006-06-01
影响因子：
3.6
通讯作者：
Morrison, Mark
中科院分区：
文献类型：
--
作者：
Kang, Seungha;Barak, Yoav;Morrison, Mark
在自然界中，许多负责植物多糖降解的厚壁菌门细菌产生称为纤维素体的多蛋白复合物，其协调糖苷水解酶组装、细菌对底物的粘附和多糖水解。纤维素体蛋白具有锚定蛋白模块，其通过与粘附蛋白模块的相互作用介导它们与支架蛋白的附着，并且已知仅糖苷水解酶和其他碳水化合物活性酶存在于多纤维素体内。我们在这里与热纤梭菌ATCC 27405的丝氨酸蛋白酶抑制剂，这是最好的承认，其构象的灵活性和协调的关键调控功能，在多细胞真核生物的丝氨酸蛋白酶抑制剂超家族的成员，也居住在纤维素体内。这些研究是第一个扩展的多纤维素体范例的蛋白质复合物组装超出糖苷水解酶和碳水化合物活性酶，并包括一个新确定的功能性厚壁菌门。
Many of the Firmicutes bacteria responsible for plant polysaccharide degradation in Nature produce a multiprotein complex called a cellulosome, which co-ordinates glycoside hydrolase assembly, bacterial adhesion to substrate and polysaccharide hydrolysis. Cellulosomal proteins possess a dockerin module, which mediates their attachment to the scaffoldin protein via its interaction with cohesin modules, and only glycoside hydrolases and other carbohydrate active enzymes were known to reside within the cellulosome. We show here with Clostridium thermocellum ATCC 27405 that members of the serpin superfamily of serine proteinase inhibitors, which are best recognized for their conformational flexibility and co-ordination of key regulatory functions in multicellular eukaryotes, also reside within the cellulosome. These studies are the first to expand the cellulosome paradigm of protein complex assembly beyond glycoside hydrolase and carbohydrate active enzymes, and to include a newly identified functionality in the Firmicutes.