EFFECT OF BERBERINE ON MYOELECTRIC ACTIVITY AND TRANSIT OF THE SMALL-INTESTINE IN RATS
EFFECT OF BERBERINE ON MYOELECTRIC ACTIVITY AND TRANSIT OF THE SMALL-INTESTINE IN RATS
复制标题
DOI：
10.1016/0016-5085(89)90519-2
复制
发表时间：
1989-06-01
期刊：
影响因子：
29.4
通讯作者：
SNINSKY, CA
中科院分区：
文献类型：
--
作者：
EAKER, EY;SNINSKY, CA
在未麻醉的大鼠中接受硫酸小檗碱（0.2、2.0和20.0 mg/kg i. p.）被查运动性通过两种方法确定：肌电活动与留置双极电极监测，和肠道传输的放射性铬（Na 51 CrO 4）的运动进行了测量。20.0 mg/kg剂量对21.8 ±. 7.0小檗碱2.0 mg/kg腹腔注射，破坏迁移性肌电复合体64.6分钟，但未观察到锋电位抑制。在最高剂量的黄连素后15和100分钟，小肠运输显著延迟（p < 0.001）。纳洛酮阻断了20.0 mg/kg小檗碱引起的峰抑制，但未能改善转运。酚妥拉明阻断尖峰抑制，并与迁移性肌电复合体的活动前沿的显着提前返回。 在转运研究中，用这种拮抗剂预处理的动物比单独注射黄连素的动物倾向于更高的几何中心。还通过各种途径（腹膜内注射、静脉内注射、口胃管饲和管腔内注射）给予小檗碱。腹膜内注射比静脉注射有效10倍。黄连素的灌胃和肠腔内给药不改变肠动力。总之，硫酸小檗碱显著抑制小肠的肌电活动和运输。这似乎部分地由阿片样物质和α-阿片样物质介导。肾上腺素能受体小檗碱的抗腹泻特性可能至少部分地通过其延迟小肠传输的能力来介导。
The motility of the small intestine in unanesthetized rats receiving berberine sulfate (0.2, 2.0, and 20.0 mg/kg i.p.) was investigated. Motility was determined by two methods: myoelectric activity was monitored with indwelling bipolar electrodes, and intestinal transit was measured by the movement of radiochromium (Na51CrO4). The 20.0-mg/kg dose caused a marked inhibition of spike activity for 21.8 .+-. 7.0 min and disrupted activity fronts of the migrating myoelectric complex for 212.3 min. Berberine, 2.0 mg/kg i.p., disrupted migrating myoelectric complexes for 64.6 min but spike inhibition was not observed. Transit of the small intestine was significantly (p < 0.001) delayed at 15 and 100 min after the highest dose of berberine. Naloxone blocked the spike inhibition noted with 20.0 mg/kg of berberine but failed to improve transit. Phentolamine blocked spike inhibition and was associated with a significantly earlier return of activity fronts of the migrating myoelectric complex. Animals pretreated with this antagonist tended toward a higher geometric center in transit studies than those injected with berberine alone. Berberine was also administered by various routes (intraperitoneal injection, intravenous injection, orogastric gavage, and intraluminal injection). An intraperitoneal injection was 10-fold more potent than an intravenous injection. Orogastric gavage and intraluminal administration of berberine did not alter intestinal motility. In summary, berberine sulfate significantly inhibits myoelectric activity and transit of the small intestine. This appears to be partially mediated by opioid and .alpha.-adrenergic receptors. The antidiarrheal properties of berberine may be mediated, at least in part, by its ability to delay small intestinal transit.