THE OLIGODENDROCYTE MYELIN GLYCOPROTEIN OF MOUSE - PRIMARY STRUCTURE AND GENE STRUCTURE
THE OLIGODENDROCYTE MYELIN GLYCOPROTEIN OF MOUSE - PRIMARY STRUCTURE AND GENE STRUCTURE
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DOI：
10.1006/geno.1993.1379
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发表时间：
1993-09-01
期刊：
影响因子：
4.4
通讯作者：
STEFANSSON, K
中科院分区：
文献类型：
--
作者：
MIKOL, DD;RONGNOPARUT, P;STEFANSSON, K
少突胶质细胞髓磷脂糖蛋白(OMgp)是一种在大脑中表达的磷脂酰肌醇连接的膜糖蛋白，在人类中是由一个基因编码的，该基因完全位于神经纤维瘤病1型(NFI)基因的内含子和相反的链上。我们从小鼠基因组文库中获得了两个不同的重叠DNA克隆，该文库包含小鼠的OMGp基因(MOMGp)。在5‘非翻译区有一个内含子，其位置与人类OMGp基因中唯一内含子的位置完全相同。小鼠内含子中的重复序列(TC)24将其分为5‘和3’片段，分别与人类基因有72%和93%的序列同源性。推导出的两个物种的未加工多肽均含有440个氨基酸，mOMgp和hOMgp一级结构的相似性表明了功能的保守性。这两个基因的编码区、非编码区和侧翼区的核苷酸序列的保守性是显著的，并增加了核苷酸序列可能起到与编码OMGp的作用不同的功能的可能性。
The oligodendrocyte-myelin glycoprotein (OMgp), a phosphatidylinositol-linked membrane glycoprotein expressed in the brain, is in man encoded by a gene that is entirely within an intron of and on the strand opposite to the neurofibromatosis type 1 (NFI) gene. We obtained two distinct overlapping DNA clones from a mouse genomic library that contain the OMgp gene from mouse (mOMgp). There is a single intron in the 5′ untranslated region in exactly the same position as the sole intron in the gene for the human OMgp (hOMgp). A repeat, (TC)24, in the mouse intron divides it into 5′ and 3′ segments that have 72 and 93% sequence identity, respectively, with the human gene. The deduced unprocessed polypeptides of both species have 440 amino acids and the similarity of the primary structures of mOMgp and hOMgp indicates conservation of function. The conservation of the nucleotide sequences of the coding, noncoding, and flanking regions of the two genes is remarkable and raises the possibility that the nucleotide sequence may serve a function that is separate from the role of encoding OMgp.