Liposomes for Specific Depletion of Macrophages from Organs and Tissues
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
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DOI：
10.1007/978-1-60327-360-2_13
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发表时间：
2010-01-01
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影响因子：
--
通讯作者：
Hendrikx, Esther
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文献类型：
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作者：
van Rooijen, Nico;Hendrikx, Esther
基于脂质体介导的小亲水分子氯膦酸盐在巨噬细胞中的内化，已经开发了脂质体介导的巨噬细胞“自杀”方法，J Leukoc Biol 62：702，1997。这种分子在循环中释放时具有非常短的半衰期，但不易穿过脂质体或细胞膜的磷脂梅耶。因此，一旦以脂质体包封形式被巨噬细胞摄取，一旦脂质体在其溶酶体磷脂酶的帮助下被消化，它将在细胞内积累。在一定的细胞内氯膦酸盐浓度下，巨噬细胞通过凋亡被消除。考虑到所选择的脂质体磷脂和氯膦酸盐对其他（非吞噬）细胞都没有毒性，该方法已证明其对各种器官中巨噬细胞亚群消耗的有效性和特异性。在一些情况下，可以通过选择氯膦酸盐脂质体的正确给药途径来获得器官特异性消耗。
A liposome mediated macrophage "suicide" approach has been developed, based on the liposome mediated internalization of the small hydrophilic molecule clodronate in macrophages J Leukoc Biol 62:702, 1997. This molecule has a very short half life when released in the circulation, but does not easily cross phospholipid Mayers of liposomes or cell membranes. As a consequence, Once ingested by a macrophage in a liposome encapsulated form, it will be accumulated within the cell as soon as the liposomes are digested with the help of its lysosomal phospholipases. At a certain intracellular clodronate concentration, the macrophage is eliminated by apoptosis. Given the fact that, neither the liposomal phospholipids chosen, nor clodronate arc toxic to other (non-phagocytic) cells, this method has proven its efficacy and specificity for depletion of macrophage Subsets in various organs. In several cases, organ specific depletion can be obtained by choosing the right administration route for the clodronate liposomes.