A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans
A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans
复制标题
DOI：
10.1126/science.1062039
复制
发表时间：
2001-09-21
期刊：
影响因子：
56.9
通讯作者：
Bass, BL
中科院分区：
文献类型：
--
作者：
Knight, SW;Bass, BL
RNA干扰（RNAi）的早期事件是起始双链RNA（dsRNA）切割成长度为21至23个核苷酸的短片段。在这里，我们描述了一个无效突变的dicer-1（dcr-1），一个基因提出编码的酶，产生这些短RNA。我们发现dcr-1（-/-）动物在某些但不是所有条件下都有RNAi缺陷。突变动物具有导致不育的生殖系缺陷，这表明dsRNA切割成短片段是正常发育中的必要事件。
An early event in RNA interference (RNAi) is the cleavage of the initiating double-stranded RNA (dsRNA) to short pieces, 21 to 23 nucleotides in length. Here we describe a null mutation in dicer-1 (dcr-1), a gene proposed to encode the enzyme that generates these short RNAs. We find that dcr-1(-/-) animals have defects in RNAi under some, but not all, conditions. Mutant animals have germ line defects that lead to sterility, suggesting that cleavage of dsRNA to short pieces is a requisite event in normal development.