Emergence of Feature-Specific Connectivity in Cortical Microcircuits in the Absence of Visual Experience
Emergence of Feature-Specific Connectivity in Cortical Microcircuits in the Absence of Visual Experience
复制标题
DOI：
10.1523/jneurosci.0875-14.2014
复制
发表时间：
2014-07-16
影响因子：
5.3
通讯作者：
Hofer, Sonja B.
中科院分区：
文献类型：
--
作者：
Ko, Ho;Mrsic-Flogel, Thomas D.;Hofer, Sonja B.
在初级视觉皮质(V1)，2/3层(L2/3)兴奋性神经元之间的连接在视觉体验开始后经历了广泛的重组，具有相似特征选择的神经元形成功能微电路(Koet al.，2011,2013)。目前尚不清楚皮层内回路的发育完善是否需要视觉经验，或者这种成熟是否由内在引导。在这里，我们将在体外通过同步全细胞记录分析的V1、L2/3神经元之间的连通性与通过双光子钙成像在体内测量的暗饲养小鼠的反应特性相关联。我们发现，在缺乏视觉体验的情况下，对定向栅格或自然电影有相似反应的神经元会优先连接。然而，在黑暗饲养的小鼠中，与自然电影视觉反应的连接性和相似性之间的关系并不像正常饲养的小鼠那样强烈。此外，黑暗养育防止了睁开眼睛后正常发生的视觉无反应神经元之间连接的丢失(Ko等人，2013年)。因此，我们的数据表明，视觉输入的缺乏并不能阻止皮层回路中出现特定功能的经常性连接；然而，视觉经验是完全微回路成熟所必需的。
In primary visual cortex (V1), connectivity between layer 2/3 (L2/3) excitatory neurons undergoes extensive reorganization after the onset of visual experience whereby neurons with similar feature selectivity form functional microcircuits (Koet al., 2011, 2013). It remains unknown whether visual experience is required for the developmental refinement of intracortical circuitry or whether this maturation is guided intrinsically. Here, we correlated the connectivity between V1 L2/3 neurons assayed by simultaneous whole-cell recordings in vitro to their response properties measured by two-photon calcium imaging in vivo in dark-reared mice. We found that neurons with similar responses to oriented gratings or natural movies became preferentially connected in the absence of visual experience. However, the relationship between connectivity and similarity of visual responses to natural movies was not as strong in dark-reared as in normally reared mice. Moreover, dark rearing prevented the normally occurring loss of connections between visually nonresponsive neurons after eye opening (Ko et al., 2013). Therefore, our data suggest that the absence of visual input does not prevent the emergence of functionally specific recurrent connectivity in cortical circuits; however, visual experience is required for complete microcircuit maturation.