Temporal changes in pH within the phagocytic vacuole of the polymorphonuclear neutrophilic leukocyte.
Temporal changes in pH within the phagocytic vacuole of the polymorphonuclear neutrophilic leukocyte.
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多形核中性粒细胞白细胞的吞噬液泡内pH的时间变化。
DOI：
10.1083/jcb.56.2.379
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发表时间：
1973-02
影响因子：
7.8
通讯作者：
Bainton, D F
中科院分区：
文献类型：
--
作者：
Jensen, M S;Bainton, D F
虽然以前的工作者已经确定，中性粒细胞（PMN）白细胞的吞噬空泡的pH值从中性到酸性的变化，转换的时间过程还没有被调查。启动本实验以研究吞噬作用后立即发生的pH变化。在大鼠中诱导腹膜渗出液; 4 h后，腹膜内注射用pH指示剂染色的酵母，并以30 s的间隔取回渗出液，并通过光学显微镜检查。结果显示，（a）在3分钟内，pH值下降到1.665，如中性红染色酵母的颜色变化所示;（B）在7-15分钟内，pH值逐渐下降到1.440，如溴甲酚绿染色酵母的颜色变化所示;（c）pH值没有下降到4以下，因为当使用溴酚蓝染色酵母时，直到24小时都没有观察到颜色变化。对于PMN白细胞在杀死和消化微生物中的功能，吞噬作用后液泡内酸度迅速增加的发现无疑是重要的，对于许多PMN白细胞颗粒酶（即，过氧化物酶和溶酶体酶）在酸性pH（约4.5）下活化。因此，在白细胞内杀微生物机制的研究中，应考虑pH值和最大pH抑制的时间变化，因为这些因素的缺陷可能导致PMN白细胞功能受损。
Although previous workers have established that the pH of the phagocytic vacuole of the polymorphonuclear (PMN) leukocyte changes from neutral to acid, the time course of conversion has not been investigated. The present experiments were initiated to study pH changes immediately after phagocytosis. Peritoneal exudates were induced in rats; 4 h later, yeast stained with pH indicators was injected intraperitoneally, and the exudate was retrieved at 30-s intervals and examined by light microscopy. Results revealed that (a) within 3 min, pH dropped to ∼6.5, as indicated by the change in color of neutral red-stained yeast; (b) within 7–15 min, pH dropped progressively to ∼4.0, as indicated by color change in bromcresol green-stained yeast; (c) pH did not fall below 4, since no color change was observed up to 24 h when bromphenol blue-stained yeast was used. The finding that intravacuolar acidity increases rapidly after phagocytosis is undoubtedly important with respect to PMN leukocyte function in killing and digesting microorganisms, for many PMN leukocyte granule enzymes (i.e., peroxidase and lysosomal enzymes) are activated at acid pH (∼4.5). It follows that temporal changes in pH and maximal pH depression should be considered in studies of intraleukocytic microbicidal mechanisms, since a defect in these factors could result in impaired PMN leukocyte function.