Involvement of Periostin in Regression of Hyaloidvascular System during Ocular Development
Involvement of Periostin in Regression of Hyaloidvascular System during Ocular Development
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DOI：
10.1167/iovs.12-9684
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发表时间：
2012-09-01
影响因子：
4.4
通讯作者：
Ishibashi, Tatsuro
中科院分区：
文献类型：
--
作者：
Arima, Mitsuru;Yoshida, Shigeo;Ishibashi, Tatsuro
目的。玻璃体血管系统(HVS)的及时消退是眼睛正常发育所必需的。虽然巨噬细胞在这一过程中发挥了关键作用，但确切的机制仍不清楚。Periostin是一种基质细胞蛋白，参与组织和血管重建。我们研究的目的是确定Periostin是否参与了HVS的退化。方法：我们使用了野生型(WT)和Periostin基因敲除(KO)小鼠。采用吲哚青绿血管造影术和异凝素B4免疫组织化学方法评价HVS回归。采用原位末端标记法对玻璃体血管内皮细胞的凋亡数进行定量。F4/80和Iba-1染色确定巨噬细胞在玻璃体中的数量和位置。免疫组织化学方法观察Periostin的定位。为确定Periostin的功能作用，采用粘附法测定人单核细胞与纤维连接蛋白的黏附程度。结果：Periostin KO小鼠中，人单核细胞与纤维连接蛋白的黏附程度推迟了人单核细胞与纤维连接蛋白的黏附程度。Periostin KO小鼠玻璃体中F4/80阳性细胞数较多。玻璃样血管附近仅有少量Iba-1阳性细胞与Periostin共染色，外周血单核细胞不与Periostin共染色。黏附实验显示Periostin可增加单核细胞与纤维连接蛋白的黏附程度。结论眼内巨噬细胞分泌的Periostin可通过增强巨噬细胞与玻璃体血管的黏附而促进HVS消退。(投资眼科VS科学。2012年；53：64956503)doi：10.1167/iovs.12-9684
PURPOSE. A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular protein involved in tissue and vascular remodeling. The purpose of our study was to determine whether periostin is involved in the HVS regression.METHODS. We used wild type (WT) and periostin knockout (KO) mice. Indocyanine green angiography and immunohistochemistry with isolectin B4 were used to evaluate the HVS regression. TUNEL-labeling was used to quantify the number of apoptotic hyaloid vascular endothelial cells. F4/80 and Iba-1 staining was performed to determine the number and location of macrophages in the vitreous. The location of periostin also was investigated by immunohistochemistry. To determine the functional role of periostin, the degree of adhesion of human monocytes to fibronectin was measured by an adhesion assay.RESULTS. The HVS regression and peak in the number of TUNEL-positive apoptotic endothelial cells were delayed in periostin KO mice. The number of F4/80 positive cells in the vitreous was higher in periostin KO mice. Only a small number of Iba-1-positive cells near the hyaloid vessels was co-stained with periostin, and peripheral blood monocytes were not stained with periostin. Adhesion assay showed that periostin increased the degree of attachment of monocytes to fibronectin.CONCLUSIONS. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the HVS regression by intensifying the adhesion of macrophages to hyaloid vessels. (Invest Ophthalmol Vis Sci. 2012;53:64956503) DOI:10.1167/iovs.12-9684