Association of the genetic markers for myocardial infarction with sudden cardiac death.
Association of the genetic markers for myocardial infarction with sudden cardiac death.
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DOI：
10.1016/j.ihj.2016.07.016
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发表时间：
2017-04
影响因子：
1.5
通讯作者：
Voevoda MI
中科院分区：
文献类型：
--
作者：
Ivanova AA;Maksimov VN;Orlov PS;Ivanoshchuk DE;Savchenko SV;Voevoda MI
探讨rs 17465637基因MIAF 3（1 q41）、rs 1376251基因TAS 2 R50（12 p13）、rs 4804611基因ZNF 627（19 p13）、rs619203基因ROS 1（6 q22）、rs 1333049（9 p21）、rs 10757278（9 p21）、rs 2549513（16 q23）、rs 499818（6p 24）与心肌梗死相关，可从国际全基因组研究中获得，在病例对照研究中发生心脏性猝死（SCD）。使用WHO标准形成SCD病例样本（n = 285）;根据性别和年龄选择对照样本（n = 421）。采用酚-氯仿抽提法从SCD患者心肌组织和对照组血液中提取DNA。使用TaqMan探针（Applied Biosystems，United States）通过实时PCR对各组进行所选SNP的基因分型。猝死组与对照组单核苷酸多态性基因型和等位基因频率差异无统计学意义。通过分组性别和年龄差异，rs 1333049、rs 10757278和rs 499818基因型频率差异均有统计学意义。rs 1333049基因型CC和rs 10757278基因型GG与男性心脏性猝死风险增加相关（分别为p = 0.019，OR = 1.7，95%CI 1.1-2.8; p = 0.011，OR = 1.8，95%CI 1.2-2.8）。rs 499818基因型AG与50岁以上女性心源性猝死风险增加相关（p = 0.009，OR = 2.4，95%CI 1.3-4.6）。rs 1333049和rs 10757278多态性与50岁以上男性SCD相关，rs 499818多态性与50岁以上女性SCD相关。
Investigate the association of rs17465637 gene MIAF3 (1q41), rs1376251 gene TAS2R50 (12p13), rs4804611 gene ZNF627 (19p13), rs619203 gene ROS1 (6q22), rs1333049 (9p21), rs10757278 (9p21), rs2549513 (16q23), rs499818 (6p24) associated with myocardial infarction available from the international genome-wide studies with sudden cardiac death (SCD) in a case–control study. A sample of SCD cases (n = 285) was formed using the WHO criteria; the control sample (n = 421) was selected according to sex and age. DNA was isolated by phenol–chloroform extraction from the myocardial tissue of SCD cases and blood of control cases. The groups were genotyped for the selected SNPs by real-time PCR using TaqMan probes (Applied Biosystems, United States). No statistically significant differences in the genotype and allelic frequencies of studied single nucleotide polymorphisms between sudden cardiac death cases and control were detectable in general group. By separating the groups of sex and age differences in the genotype frequencies of rs1333049, rs10757278 and rs499818 are statistical significance. Genotypes CC of rs1333049 and GG of rs10757278 are associated with an increased sudden cardiac death risk in men (p = 0.019, OR = 1.7, 95% CI 1.1–2.8; p = 0.011, OR = 1.8, 95% CI 1.2–2.8, respectively). Genotype AG of rs499818 is associated with an increased sudden cardiac death risk in the women over 50 years old (p = 0.009, OR = 2.4, 95% CI 1.3–4.6). Polymorphisms rs1333049 and rs10757278 are associated with SCD in men and rs499818 in the women aged over 50 years.