Chlorophyll Supplementation in Early Life Prevents Diet-Induced Obesity and Modulates Gut Microbiota in Mice
Chlorophyll Supplementation in Early Life Prevents Diet-Induced Obesity and Modulates Gut Microbiota in Mice
复制标题
生命早期补充叶绿素可预防饮食引起的肥胖并调节小鼠肠道微生物群
DOI：
10.1002/mnfr.201801219
复制
发表时间：
2019-08-05
影响因子：
5.2
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Li, Yuanyuan;Cui, Yu;Zhang, Yan
范围目前儿童超重和肥胖的发生率很高，这凸显了在体重增加之前的早期预防策略的迫切需要。方法和结果在这项研究中，使用4周龄的C57 BL/6 J雄性小鼠模型，通过高脂饮食（HFD）诱导肥胖，研究了在生命早期补充叶绿素的减轻肥胖特性。目前的研究发现，在生命早期补充叶绿素可以有效地延缓体重增加，改善葡萄糖耐量，以及减少HFD喂养小鼠的低度炎症。因此，16 S rRNA的高通量测序表明，叶绿素补充显著逆转了HFD诱导的肠道生态失调，如叶绿素引入时厚壁菌与拟杆菌比率降低所证明的。此外，在叶绿素喂养的小鼠中，布劳特氏菌和诺兰克氏菌_类杆菌_S24 - 7_群的丰度增加，以及乳球菌和乳杆菌的丰度减少，使得叶绿素对肠道微生物群组成的改变可能是膳食叶绿素减轻肥胖相关指标的主要因素。总之，这些结果表明，在生命早期摄入叶绿素可以促进健康的体重管理，并对以后的生活中潜在的肥胖产生有益的影响。
Scope The high rates of overweight and obesity, currently occurring in children, underline the urgent need for preventive strategies in early life before excess weight is gained. Methods and results In this study, the alleviating obesity property of chlorophyll supplementation in early life is investigated using a 4-week-old C57BL/6J male mice model with obesity induced by high-fat diet (HFD). The present study finds that chlorophyll supplementation in early life can effectively retard body weight gain, improve glucose tolerance, as well as reduce low-grade inflammation in HFD-fed mice. Consequently, high-throughput sequencing of the 16S rRNA demonstrates that chlorophyll supplementation significantly reversed the HFD-induced gut dysbiosis, as evidenced by the decreased Firmicutes-to-Bacteroidetes ratios as chlorophyll is introduced. Furthermore, in chlorophyll-fed mice, the increased abundance of Blautia and norank_f_Bacteroidales_S24-7_group, and the decreased abundance of Lactococcus and Lactobacillus, bring about the possibility that chlorophyll's alterations to the gut microbiota composition would be the main factors for dietary chlorophyll alleviating obesity-related indexes. Conclusion Taken together, these results reveal that the intake of chlorophyll in early life may propel healthy weight management and produce beneficial effects against potential obesity in later life.