Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 or early S phase with cycloheximide or puromycin.
Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 or early S phase with cycloheximide or puromycin.
复制标题
用放线菌酮或嘌呤霉素处理 G1 期或早期 S 期的同步化中国仓鼠细胞中 DNA 合成的抑制。
DOI：
10.1016/0014-4827(72)90435-1
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发表时间：
1972
影响因子：
3.7
通讯作者：
W. C. Dewey
中科院分区：
文献类型：
--
作者：
D. P. Highfield;W. C. Dewey
用蛋白质合成抑制剂放线菌酮（CHM，5 μg/ml）或嘌呤霉素（PUR，50 μg/ml）处理同步化中国仓鼠卵巢细胞，观察G1期和S早期细胞后DNA合成的起始和持续延迟。虽然蛋白质的合成被抑制超过95%，在10或30分钟后，管理CHM或PUR，分别位于早期S的细胞可以合成DNA（H3 HTdR）在一个大大降低的速度，和细胞位于G1在55分钟内的S可以启动DNA合成在6小时的治疗期间。当处理终止时，RNA和蛋白质合成几乎立即恢复，但位于早期S期的细胞中的DNA合成的正常速率仅在RNA和蛋白质合成持续约3小时后才达到。这3小时的延迟也被观察到的DNA合成的起始在细胞中被困在晚G1之前的标记位于55分钟之前S。这些结果与我们以前的研究相结合表明，除了需要伴随的组蛋白合成外，还有不稳定的因子，可能是特异性蛋白质，其平均寿命为2-3小时，这是必要的：（1）DNA合成以正常速率继续进行;（2）如果G1细胞位于S前55分钟以上，则G1细胞向S的进展。
The delay in initiation and continuation of DNA synthesis after treatments during G 1 and early S with cycloheximide (CHM, 5 μg/ml) or puromycin (PUR, 50 μg/ml), inhibitors of protein synthesis, has been studied in synchronous Chinese hamster ovary cells. Although protein synthesis is inhibited by more than 95% within 10 or 30 min after administering CHM or PUR, respectively, cells located in early S can synthesize DNA (incorporate3HTdR) at a greatly reduced rate, and cells located in G1 within 55 min of S can initiate DNA synthesis during the 6-h treatment period. When the treatment is terminated, RNA and protein synthesis resume almost immediately, but the normal rate of DNA synthesis in cells located in early S phase is achieved only after RNA and protein synthesis have continued for about 3-h. This 3-h delay also is observed for the initiation of DNA synthesis in cells trapped in late G1 prior to a marker located 55 min before S. These results considered with our previous studies suggest that, in addition to the requirement for concomitant histone synthesis, there are labile factors, probably specific proteins, having a mean life of 2–3 h which are necessary (1) for the continuation of DNA synthesis at the normal rate; (2) for the progression of G1 cells into S if they are located more than 55 min prior to S.