Gene expression profiling of the rat endometriosis model
Gene expression profiling of the rat endometriosis model
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DOI：
10.1111/j.1600-0897.2007.00507.x
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Suzuki, Mitsuaki
中科院分区：
文献类型：
--
作者：
Konno, Ryo;Fujiwara, Hiroyuki;Suzuki, Mitsuaki
问题：为了研究子宫内膜异位症的分子机制，我们分析了大鼠子宫内膜异位症模型的基因表达谱。研究方法：采用子宫自体移植术在8周龄雌性sd大鼠腹腔内建立子宫内膜异位症模型。以正常子宫组织作为对照。采用cDNA微阵列分析、实时定量RT-PCR和免疫组化方法比较子宫内膜异位症病变与正常子宫组织的基因表达。结果：与正常子宫组织相比，子宫内膜异位症病变中有71个基因表达上调，45个基因表达下调。上调的基因包括编码细胞因子、趋化因子、生长因子和细胞粘附分子的基因。骨桥蛋白、Lyn、Vav1、Runx1和l -选择素在子宫内膜异位症病变中的转录本水平分别比异位子宫内膜样品高130倍、10倍、10倍、12倍和46倍。结论：骨桥蛋白、Lyn、Vav1、Runx1、l -选择素在子宫内膜异位症发病中起重要作用。
Problem:To investigate the molecular mechanism of endometriosis, gene expression profiling was analyzed in a rat endometriosis model.Method of study:An endometriosis model was induced by uterine autotransplantation in the peritoneal cavity on a female-SD rat (8 weeks old). As control samples, the normal uterine tissues were used. The gene expression was compared between endometriotic lesions and normal uterine tissues by cDNA microarray analysis, quantitative real time RT-PCR and immunohistochemistry.Results:The expression of 71 genes was upregulated and that of 45 genes was downregulated in the endometriotic lesions compared to normal uterine tissues. The upregulated genes included genes encoding cytokines, chemokines, growth factors and cell adhesion molecules. The levels of transcripts of osteopontin, Lyn, Vav1, Runx1, and L-selectin in the endometriotic lesions were 130, 10, 10, 12 and 46-fold higher than the respective levels in the eutopic endometrial samples.Conclusion:The results suggest that osteopontin, Lyn, Vav1, Runx1, and L-selectin play important roles in the pathogenesis of endometriosis.