Genome-wide analysis of the R2R3-MYB transcription factor gene family in sweet orange (Citrus sinensis)
Genome-wide analysis of the R2R3-MYB transcription factor gene family in sweet orange (Citrus sinensis)
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DOI：
10.1007/s11033-014-3563-1
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发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Chaoyang;Wang, Xia;Xu, Qiang
MYB转录因子是植物基因组中最大的基因家族之一。甜橙是世界上最重要的果树作物之一，目前已完成基因组测序。这为从全基因组角度研究甜橙MYB基因的组织和进化特征提供了机会。在本研究中，我们在甜橙基因组中鉴定了100个R2R3-MYB基因。对该基因家族进行了系统发育、基因结构、染色体定位和表达谱分析。根据序列相似性和系统发育将这100个基因分为29个亚家族，高度保守的外显子/内含子结构和基序组成也很好地支持了分类。甜橙及其近缘植物拟南芥、可可和番木瓜MYB基因家族的系统发育比较表明，在进化过程中存在功能分化。表达谱显示甜橙R2R3-MYB基因表现出不同的时间和空间表达模式。我们的分析表明，甜橙MYB基因可能在不同的植物生物学过程中发挥重要作用，其中一些可能与柑橘果实的品质有关。这些结果将为未来甜橙MYB基因家族的功能分析提供有用的信息。
MYB transcription factor represents one of the largest gene families in plant genomes. Sweet orange (Citrus sinensis) is one of the most important fruit crops worldwide, and recently the genome has been sequenced. This provides an opportunity to investigate the organization and evolutionary characteristics of sweet orange MYB genes from whole genome view. In the present study, we identified 100 R2R3-MYB genes in the sweet orange genome. A comprehensive analysis of this gene family was performed, including the phylogeny, gene structure, chromosomal localization and expression pattern analyses. The 100 genes were divided into 29 subfamilies based on the sequence similarity and phylogeny, and the classification was also well supported by the highly conserved exon/intron structures and motif composition. The phylogenomic comparison of MYB gene family among sweet orange and related plant species, Arabidopsis, cacao and papaya suggested the existence of functional divergence during evolution. Expression profiling indicated that sweet orange R2R3-MYB genes exhibited distinct temporal and spatial expression patterns. Our analysis suggested that the sweet orange MYB genes may play important roles in different plant biological processes, some of which may be potentially involved in citrus fruit quality. These results will be useful for future functional analysis of the MYB gene family in sweet orange.