The estrogen-responsive 110K and 74K rat uterine secretory proteins are structurally related to complement component C3.
The estrogen-responsive 110K and 74K rat uterine secretory proteins are structurally related to complement component C3.
复制标题
雌激素反应性 110K 和 74K 大鼠子宫分泌蛋白在结构上与补体成分 C3 相关。
DOI：
10.1016/0006-291x(89)92807-6
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
DeSombre,ER
中科院分区：
文献类型：
--
作者：
Kuivanen,PC;Capulong,RB;Harkins,RN;DeSombre,ER
雌激素刺激大鼠子宫内特异性分泌蛋白的合成。在这里，我们表明，其中两个，相对分子量为110，000（110 K）和74，000（74 K）的多肽，在结构上与补体的第三种成分C3相关，补体是一种糖蛋白，在调节补体介导的炎症和免疫反应中起着重要作用。相似性是基于以下观察结果：（1）74K多肽的NH 2端氨基酸序列与小鼠C3的β链具有序列同源性，（2）在有和无还原剂的情况下比较110K和74K多肽的电泳迁移率表明，它们是Mr为180，000的蛋白质的二硫键连接亚基，（3）天然蛋白质与大鼠C3特异性抗体具有免疫反应性，和（4）两种多肽与大鼠C3的抗体免疫沉淀。
Estrogens stimulate the synthesis of specific secretory proteins in the rat uterus. Here we show that two of these, polypeptides of relative molecular weight 110,000 (110K) and 74,000 (74K), are structurally related to C3, the third component of complement, a glycoprotein that plays a central role in regulating complementmediated inflammatory and immune responses. The similarities were based on the observations that (1) NH2-terminal amino acid sequence of the 74K polypeptide showed sequence homology with the β chain of mouse C3, (2) comparison of the electrophoretic mobilities of the 110K and 74K polypeptides in the presence and absence of reducing agents revealed that they were disulfide-linked subunits of a protein of Mr ∼ 180,000, (3) the native protein was immunoreactive with antibodies specific for rat C3, and (4) both polypeptides were immunoprecipitated with antibodies to rat C3.