SOMATOLACTIN, A NOVEL PITUITARY PROTEIN - PURIFICATION AND PLASMA-LEVELS DURING REPRODUCTIVE MATURATION OF COHO SALMON
SOMATOLACTIN, A NOVEL PITUITARY PROTEIN - PURIFICATION AND PLASMA-LEVELS DURING REPRODUCTIVE MATURATION OF COHO SALMON
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DOI：
10.1677/joe.0.1330393
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发表时间：
1992-06-01
影响因子：
4
通讯作者：
DICKHOFF, WW
中科院分区：
文献类型：
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作者：
RANDWEAVER, M;SWANSON, P;DICKHOFF, WW
从银鲑（Oncorhynchus kisutch）中分离得到一种新发现的GH/催乳素家族的垂体蛋白——生长肌动素（somatactin， SL）。将抗体培养到纯化的coho SL，并建立并验证了同源放射免疫测定法。该试验对SL具有特异性，与银鲑GH、促性腺激素I和II无交叉反应，与催乳素的交叉反应小于0.2%。对银鲑、奇努克鲑鱼和虹鳟鱼等Oncorhynchus物种的血浆和垂体提取物进行连续稀释，与银鲑SL标准曲线平行。大西洋鲑鱼（Salmo salar）血浆稀释后的位移曲线与标准平行，而脑垂体提取物不与标准平行。在整个生殖成熟的最后一年，测量了银鲑的血浆SL水平。在性腺生长期间，血浆SL水平升高，且与雌性雌二醇水平和雄性11-酮睾酮水平高度相关。在两性的最终成熟和产卵时观察到SL的峰值水平。据推测，SL可能调节生殖的某些生理方面。
Somatolactin (SL), a newly discovered fish pituitary protein belonging to the GH/prolactin family, was isolated from coho salmon (Oncorhynchus kisutch). Antibodies were raised to purified coho SL, and a homologous radioimmunoassay was developed and validated. The assay was specific for SL as indicated by the absence of cross-reactivity with coho salmon GH, gonadotrophins I and II and less than 0.2% cross-reaction to prolactin. Serial dilutions of plasma and pituitary extracts from Oncorhynchus species including coho salmon, chinook salmon and rainbow trout were parallel to the coho salmon SL standard curve. Displacement curves for dilutions of Atlantic salmon (Salmo salar) plasma, but not pituitary extract were parallel to the standards. Plasma levels of SL were measured in coho salmon throughout the final year of reproductive maturation. During the period of gonadal growth, plasma SL levels increased and were highly correlated to oestradiol levels in females and 11-ketotestosterone levels in males. Peak levels of SL were observed at the time of final maturation and spawning in both sexes. It is hypothesized that SL may regulate some physiological aspect of reproduction.