Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts
Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts
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DOI：
10.1038/35008607
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发表时间：
2000-04-01
影响因子：
21.3
通讯作者：
Geiger, B
中科院分区：
文献类型：
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作者：
Zamir, E;Katz, M;Geiger, B
在这里，我们使用延时显微镜分析表达两种不同的细胞骨架蛋白之一的细胞基质粘附，paxillin或tensin，绿色荧光蛋白（GFP）标记。使用GFP-paxillin分析焦点接触和GFP-tensin研究纤维粘附显示，两种类型的主要粘附都是高度动态的，小的焦点接触经常移位，通过向心延伸和向周收缩，平均速度为19微米/小时。纤维粘连产生于固定的局点接触的中间端，包含α (5) β(1)整合素和紧张素，但不包含其他局点接触成分，并与纤维连接蛋白原纤维相关联。纤维粘连以每小时18微米的平均速率以肌动球蛋白依赖的方式向心转移。我们提出了一个动态模型，用于调节细胞-基质粘附以及在焦点接触和纤维粘附之间的转换，并具有基质变形的能力作为机械开关。
Here we use time-lapse microscopy to analyse cell-matrix adhesions in cells expressing one of two different cytoskeletal proteins, paxillin or tensin, tagged with green fluorescent protein (GFP), Use of GFP-paxillin to analyse focal contacts and GFP-tensin to study fibrillar adhesions reveals that both types of major adhesion are highly dynamic, Small focal contacts often translocate, by extending centripetally and contracting peripherally, at a mean rate of 19 micrometres per hour. Fibrillar adhesions arise from the medial ends of stationary focal contacts, contain alpha(5)beta(1) integrin and tensin but not other focal-contact components, and associate with fibronectin fibrils, Fibrillar adhesions translocate centripetally at a mean rate of 18 micrometres per hour in an actomyosin-dependent manner. We propose a dynamic model for the regulation of cell-matrix adhesions and for transitions between focal contacts and fibrillar adhesions, with the ability of the matrix to deform functioning as a mechanical switch.