UV-irradiated DNA matrixes selectively bind endocrine disruptors with a planar structure
UV-irradiated DNA matrixes selectively bind endocrine disruptors with a planar structure
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DOI：
10.1021/es011008r
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发表时间：
2002-03-01
影响因子：
11.4
通讯作者：
Nishi, N
中科院分区：
文献类型：
--
作者：
Yamada, M;Kato, K;Nishi, N
DNA具有独特的化学性质，很容易从鲑鱼和贝类生殖腺中纯化出来。DNA几乎没有商业用途，通常作为工业废物被丢弃。最近，我们通过紫外光照射制备了水不溶性和抗核酸酶的DNA膜。DNA膜去除DNA嵌入化合物。本研究采用紫外光照射法将双链DNA固定在多孔玻璃微珠上，制备了DNA固定化玻璃微珠柱。DNA固定化柱有效地积累了更多的DNA嵌入材料比DNA膜。DNA固定柱结合内分泌干扰物与平面结构，如二恶英衍生物，多氯联苯（PCB）衍生物，和苯并[a]芘。双酚A和己烯雌酚，缺乏平面结构，没有绑定到DNA固定柱。这些结果表明，DNA固定化玻璃珠柱可以选择性地去除化合物与平面结构的嵌入到双链DNA。通过紫外光照射制备的DNA固定化玻璃珠和DNA膜具有作为医学、工程和环境应用的有用生物材料的潜力。
DNA has unique chemical properties and is readily purified from salmon milts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. Recently, we prepared water-insoluble and nuclease-resistant DNA-films by UV irradiation. The DNA-films removed DNA-intercalating compounds. Here, we immobilized double-stranded DNA onto porous glass beads by UV irradiation and prepared DNA-immobilized glass bead columns. The DNA-immobilized columns effectively accumulated more DNA-intercalating materials than the DNA-films. The DNA-immobilized columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, and benzo[a]pyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized glass bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized glass beads and DNA-films prepared by UV irradiation have the potential to serve as useful biomaterials for medical, engineering, and environmental applications.