AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
AMPK activation, eEF2 inactivation, and reduced protein synthesis in the cerebral cortex of hibernating chipmunks
复制标题
冬眠花栗鼠大脑皮层 AMPK 激活、eEF2 失活和蛋白质合成减少
DOI：
10.1038/s41598-019-48172-7
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sekijima T and Takei N
中科院分区：
文献类型：
--
作者：
Yamada S;Kamata T;Nawa H;Sekijima T and Takei N
在冬眠期间，哺乳动物细胞暴露于严重的环境压力源，如低温、低氧供应和葡萄糖缺乏。为了适应这些条件，细胞代谢率显著降低。amp活化蛋白激酶（AMPK）感知细胞能量状态并调节代谢。因此，我们在冬眠的花栗鼠的几个大脑区域和外周组织中检测了AMPK信号。真核延伸因子2 （eEF2）是AMPK的下游靶点。冬眠的花栗鼠的大脑皮层中eEF2的磷酸化，表明其失活。研究表明，冬眠的花栗鼠大脑皮层中ampk -哺乳动物雷帕霉素复合物1-eEF2信号的顺序调控发生改变，蛋白质合成能力降低。
During hibernation, mammalian cells are exposed to severe environmental stressors such as low temperature, lowered O2supply, and glucose deficiency. The cellular metabolic rate is markedly reduced for adapting to these conditions. AMP-activated protein kinase (AMPK) senses the cellular energy status and regulates metabolism. Therefore, we examined AMPK signaling in several brain regions and peripheral tissues in hibernating chipmunk. Eukaryotic elongation factor 2 (eEF2) is a downstream target of AMPK. Phosphorylation of eEF2, indicating its inactivation, is enhanced in the cerebral cortex of hibernating chipmunks. The study indicated that the sequential regulation of AMPK-mammalian target of rapamycin complex 1-eEF2 signaling was altered and protein synthesis ability was reduced in the cerebral cortex of hibernating chipmunks.