Identification of a novel mouse membrane-bound family 1 glycosidase-like protein, which carries an atypical active site structure
Identification of a novel mouse membrane-bound family 1 glycosidase-like protein, which carries an atypical active site structure
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DOI：
10.1016/s0167-4781(02)00281-6
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发表时间：
2002-07-19
期刊：
影响因子：
--
通讯作者：
Nabeshima, Y
中科院分区：
文献类型：
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作者：
Ito, S;Fujimori, T;Nabeshima, Y
我们已经鉴定出一种新的小鼠基因 klph（Klotho-LPH 相关蛋白；其中 LPH 代表乳糖酶-根皮苷水解酶），它编码一种新的哺乳动物 I 族糖苷酶样蛋白。 KLPH 是一种推定的 I 型膜蛋白，由 N 端信号序列、糖苷酶结构域、跨膜区和短胞质尾组成。尽管其总体结构与其他 I 族糖苷酶相似，但该蛋白质中用于酸碱催化剂的谷氨酸并不保守。 klph mRNA 主要在肾脏和皮肤中表达。表位标记的 KLPH 定位于培养细胞内质网的核周管状网络结构。 (C) 2002 Elsevier Science B.V. 保留所有权利。
We have identified a novel mouse gene klph (Klotho-LPH related protein; where LPH stands for lactase-phlorizin hydrolase) that encodes a novel mammalian family I glycosidase-like protein. KLPH was a putative type I membrane protein that consists of N-terminal signal sequence, glycosidase domain, transmembrane region and short cytoplasmic tail. Despite its overall structural similarity to other family I glycosidases, the glutamic acid for the acid-base catalyst was not conserved in this protein. klph mRNA was predominantly expressed in the kidney and skin. Epitope-tagged KLPH was localized to the perinuclear tubular network structure of the endoplasmic reticulum in cultured cells. (C) 2002 Elsevier Science B.V. All rights reserved.