Maternal Stress Affects Postnatal Growth and the Pituitary Expression of Prolactin in Mouse Offspring
Maternal Stress Affects Postnatal Growth and the Pituitary Expression of Prolactin in Mouse Offspring
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DOI：
10.1002/jnr.22550
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发表时间：
2011-03-01
影响因子：
4.2
通讯作者：
Watanabe, Kenji
中科院分区：
文献类型：
--
作者：
Gao, Pengfei;Ishige, Atsushi;Watanabe, Kenji
母亲的压力会对后代施加长期的精神和躯体压力，这种压力会一直持续到成年。然而，关于母体应激对幼崽出生后生长发育的影响还没有广泛的报道。在这项研究中，我们发现哺乳期母体束缚应激(IS)导致雄性小鼠后代体重降低，并在断奶后持续。尽管可以免费获得食物，但它会导致母体营养不良，并降低母亲和幼崽的血清胰岛素样生长因子-1(IGF-1)水平。对仔鼠脑垂体前叶激素基因表达的分析表明，IS可降低生长激素(GH)和催乳素(PRL)，但不影响其他激素。垂体转录因子PIT1及其异构体PITX2的表达减少，而对早期垂体发育至关重要的PROP1的表达没有变化。免疫组织化学显示垂体PRL蛋白表达减少。这些结果表明，产后母亲的压力对后代的体重有持续的影响。子代脑下垂体中PRL表达的减少可能在这些影响中起到了作用。(C)2010年Wiley-Liss公司
Maternal stress exerts long-lasting psychiatric and somatic on offspring, which persist into adulthood. However, the effect of maternal stress on the postnatal growth of pups has not been widely reported. In this study, we found that maternal immobilization stress (IS) during lactation resulted in low body weight of male mouse offspring, which persisted after weaning. Despite free access to chow, IS induced maternal malnutrition and decreased the serum insulin-like growth factor-1 (IGF-1) levels in the mothers and in the pups. mRNA expression analysis of anterior pituitary hormones in the pups revealed that growth hormone (GH) and prolactin (PRL), but no other hormones, were decreased by IS. Expression of the pituitary transcription factor PIT1 and isoforms of PITX2, which are essential for the development and function of GH-producing somatotropes and PRL-producing lactotropes, was decreased, whereas that of PROP1, which is critical for the earlier stages of pituitary development, was unchanged. Immunohistochemistry also showed a decrease in pituitary PRL protein expression. These results suggest that stress in a postpartum mother has persistent effects on the body weight of the offspring. Reduced PRL expression in the offspring's pituitary gland may play a role in these effects. (C) 2010 Wiley-Liss, Inc.