Vision enhancement system for detection of oral cavity neoplasia based on autofluorescence
Vision enhancement system for detection of oral cavity neoplasia based on autofluorescence
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DOI：
10.1002/hed.10381
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发表时间：
2004-03-01
影响因子：
2.9
通讯作者：
Richards-Kortum, R
中科院分区：
文献类型：
--
作者：
Svistun, E;Alizadeh-Naderi, R;Richards-Kortum, R
背景口腔鳞状细胞癌（SCC）的早期发现可以提高生存率。通常很难用标准的白色光照明区分肿瘤和良性病变。我们评估了一种利用替代对比源组织自体荧光的技术是否能改善视觉检查。视觉上观察新鲜切除的口腔组织的自体荧光，并在针对人类视觉系统和组织荧光特性的响应而优化的特定激发/发射波长组合下拍照。对于每个波长组合指示感知的肿瘤边缘。从每个标本的几个部位获得打孔活检。通过组织病理学诊断与视觉印象的相关性来评估敏感性和特异性。在400 nm处照射并在530 nm处观察可获得最佳结果。在这里，敏感性和特异性分别为91%和86%，在正常组织的肿瘤区分。与白色光检查相比，其敏感性和特异性分别为75%和43%。口腔自发荧光可以很容易地被人眼真实的观察到。自体荧光的视觉检查增强了新鲜组织切除中正常和肿瘤口腔粘膜之间的感知对比度。(C)2004 Wiley Periodicals，Inc.
Background. Early detection of squamous cell carcinoma (SCC) in the oral cavity can improve survival. It is often difficult to distinguish neoplastic and benign lesions with standard white light illumination, We evaluated whether a technique that capitalizes on an alternative source of contrast, tissue autofluorescence, improves visual examination.Methods. Autofluorescence of freshly resected oral tissue was observed visually and photographed at specific excitation/emission wavelength combinations optimized for response of the human visual system and tissue fluorescence properties. Perceived tumor margins were indicated for each wavelength combination. Punch biopsies were obtained from several sites from each specimen. Sensitivity and specificity were evaluated by correlating histopathologic diagnosis with visual impression.Results. Best results were achieved with illumination at 400 nm and observation at 530 nm. Here, sensitivity and specificity were 91% and 86% in discrimination of normal tissue from neoplasia. This compares favorably with white light examination, in which sensitivity and specificity were 75% and 43%.Conclusions. Oral cavity autofluorescence can be easily viewed by the human eye in real time. Visual examination of autofluorescence enhances perceived contrast between normal and neoplastic oral mucosa in fresh tissue resections. (C) 2004 Wiley Periodicals, Inc.