Circulating stromal osteonectin-positive progenitor cells and stenotic coronary atherosclerosis
Circulating stromal osteonectin-positive progenitor cells and stenotic coronary atherosclerosis
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DOI：
10.1139/y07-001
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发表时间：
2007-03-01
影响因子：
2.1
通讯作者：
Smirnov, Vladimir N.
中科院分区：
文献类型：
--
作者：
Gabbasov, Zufar A.;Agapov, Alexander A.;Smirnov, Vladimir N.
采用流式细胞术检测冠心病患者外周血中携带成骨分化标志物骨连接素（ON）的循环基质祖细胞水平。99例CAD患者纳入研究。所有患者冠状动脉造影均显示至少2支冠状动脉或其主要分支严重狭窄。对照组包括8例非冠心病患者和19例健康志愿者。在对照组患者中，血管造影未发现冠状动脉床病变。自行车负荷试验证实志愿者无CAD。在各种淋巴细胞样细胞群中检查血液中ON阳性细胞的含量。发现无冠状动脉狭窄患者血液中具有CD 41阳性的ON+淋巴细胞样细胞的数量（0.27% +/-0.11%，平均值+/- SD）与健康志愿者的相应值（0.26% +/-0.07%，p = 0.94）没有显著差异。在CAD患者中，这些ON+细胞的百分比为1.01% +/-0.49%，显著高于健康志愿者（p < 0.0001）和无CAD患者（p < 0.0001）的血液。血液中高含量的ON+淋巴细胞样细胞和CD 41阳性细胞可作为动脉粥样硬化的无创性标志物。
The level of circulating stromal progenitor cells carrying osteonectin (ON), a marker of osteogenic differentiation, was evaluated by flow cytometry in blood of patients with coronary artery disease (CAD). Ninety-nine patients with CAD were included into the study. Coronary angiography of all patients showed critical stenosis of at least 2 coronary arteries or their major branches. The control groups included 8 patients without CAD and 19 healthy volunteers. In control patients, no lesions of the coronary bed were found by angiography. The absence of CAD in the volunteers was confirmed by bicycle stress test. The content of ON-positive cells in blood was examined in various populations of lymphocyte-like cells. It was found that the number of ON+ lymphocyte-like cells with CD41 positivity in blood of patients without coronary stenosis (0.27% +/- 0.11%, mean +/- SD) did not differ significantly from corresponding value in healthy volunteers (0.26% +/- 0.07%, p = 0.94). In CAD patients, the percent of these ON+ cells was 1.01% +/- 0.49% and was significantly higher than in blood of healthy volunteers (p < 0.0001) and patients without CAD (p < 0.0001). High content of ON+ lymphocyte-like cells with CD41 positivity in blood may serve as noninvasive marker of arterial atherosclerosis.