Role for antisense RNA in regulating circadian clock function in Neurospora crassa
Role for antisense RNA in regulating circadian clock function in Neurospora crassa
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DOI：
10.1038/nature01427
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发表时间：
2003-02-27
期刊：
影响因子：
64.8
通讯作者：
Crosthwaite, SK
中科院分区：
文献类型：
--
作者：
Kramer, C;Loros, JJ;Crosthwaite, SK
反义RNA在真核生物中的普遍性尚不清楚，只有少数天然存在的反义转录物被赋予了功能（1-4）。然而，最近鉴定出大量推定的反义转录物（5），这加强了反义RNA可能影响比以前认为的更广泛的过程的观点。在这里，我们表明，在模式生物粗糙脉孢菌夹带的昼夜节律钟，这是关键的基因及其产品的正确的时间表达，部分控制由时钟组件位点产生的反义RNA。在野生型菌株中，反义频率（frq）转录物的水平在黑暗中与有义frq转录物反相循环，并且可被光诱导。在光诱导反义frq RNA被消除的突变株中，内部时钟的时间相对于野生型株被延迟，并且光对时钟的重置被改变。这些数据提供了一个意想不到的反义RNA和昼夜节律之间的联系，并提供了一个新的例子，真核细胞的过程中自然发生的反义RNA的调节。
The prevalence of antisense RNA in eukaryotes is not known and only a few naturally occurring antisense transcripts have been assigned a function(1-4). However, the recent identification of a large number of putative antisense transcripts(5) strengthens the view that antisense RNAs might affect a wider variety of processes than previously thought. Here we show that in the model organism Neurospora crassa entrainment of the circadian clock, which is critical for the correct temporal expression of genes and their products, is controlled partly by an antisense RNA arising from a clock component locus. In a wild-type strain, levels of antisense frequency (frq) transcripts cycle in antiphase to sense frq transcripts in the dark, and are inducible by light. In mutant strains in which the induction of antisense frq RNA by light is abolished, the time of the internal clock is delayed relative to the wild-type strain, and resetting of the clock by light is altered. These data provide an unexpected link between antisense RNA and circadian timing and provide a new example of a eukaryotic cellular process regulated by naturally occurring antisense RNA.