Characterization of rabbit stromal fibroblasts derived from red and yellow bone marrow.
Characterization of rabbit stromal fibroblasts derived from red and yellow bone marrow.
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源自红色和黄色骨髓的兔基质成纤维细胞的表征。
DOI：
10.1084/jem.163.2.400
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stern,R
中科院分区：
文献类型：
--
作者：
Bainton,DF;Maloney,MA;Patt,HM;Stern,R
从红骨髓和黄骨髓传代培养的兔基质成纤维细胞植入肾包膜下形成小骨，其血细胞构成反映了用于培养的骨髓的细胞构成。虽然培养的红骨髓细胞和黄骨髓细胞在精细结构外观上是相似的，但它们在α-萘基丁酸酯酶的酶含量方面存在显著差异，该酶含量仅在来自黄骨髓的细胞中丰富。其他观察者（20，21）提出，基质成纤维细胞是前脂肪细胞，这一数据表明，来自黄骨髓的那些具有更分化的脂肪细胞的表型。另一方面，红骨髓和黄骨髓来源的成纤维细胞的前胶原合成谱没有差异。两种类型的成纤维细胞分泌III型前胶原作为主要种类，I/III比为1：3;相反，兔真皮成纤维细胞具有I型前胶原的显著峰。红骨髓和黄骨髓来源的基质细胞在前胶原合成方面的相似性表明，细胞外基质的胶原部分并不是其造血能力内在差异的唯一基础。
Rabbit stromal fibroblasts subcultured from red and yellow bone marrow and implanted beneath the renal capsule form ossicles the hemic cellularity of which mirrors the cellularity of the marrow used for culture. Although the cultured red and yellow marrow cells are similar in fine-structural appearance, they differ strikingly in enzymatic content of alpha-naphthylbutyrate esterase, which is abundant only in the cells derived from yellow marrow. Other observers (20, 21) have proposed that stromal fibroblasts are preadipocytes, and this data suggests that those derived from yellow marrow have the phenotype of more differentiated adipocytes. On the other hand, fibroblasts derived from red and yellow bone marrow show no differences in their profiles of procollagen synthesis. Both types of fibroblasts secrete type III procollagen as the major species, with a I/III ratio of 1:3; in contrast, rabbit dermal fibroblasts have a prominent peak of type I procollagen. The similarity of stromal cells derived from red and yellow bone marrow in procollagen synthesis suggests that the collagen part of the extracellular matrix is not the only basis for their intrinsic difference in capacity for hematopoiesis.