Tumor necrosis factor-α alters calcium handling and increases arrhythmogenesis of pulmonary vein cardiomyocytes
Tumor necrosis factor-α alters calcium handling and increases arrhythmogenesis of pulmonary vein cardiomyocytes
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DOI：
10.1016/j.lfs.2007.02.029
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发表时间：
2007-04-17
期刊：
影响因子：
6.1
通讯作者：
Chen, Shih-Ann
中科院分区：
文献类型：
--
作者：
Lee, Shih-Huang;Chen, Yao-Chang;Chen, Shih-Ann
炎症和钙稳态异常在心房颤动中起重要作用。肿瘤坏死因子α。(TNFα），一种促炎细胞因子，可诱导心律失常。肺静脉（PV）是引发阵发性房颤的关键。本研究旨在研究TNF α是否可以改变PV心肌细胞的钙处理和促凋亡活性。我们采用全细胞膜片钳和吲哚荧光比率技术，研究了与TNF α（25 ng/ml）孵育7-10 h的兔单个PV心肌细胞（对照组）的动作电位、离子电流和细胞内钙。通过共聚焦显微照片和Western印迹评价对照和TNF α处理的PV心肌细胞中肌浆网ATP酶的表达。我们发现对照组（n = 45）和TNF α处理组（n = 28）PV心肌细胞的自发搏动率相似。与对照PV心肌细胞相比，TNF α处理的PV心肌细胞具有显著更大的延迟后除极幅度（6.0 +/- 1.7 vs. 2.6 +/- 0.8 mV，P
Inflammation and abnormal calcium homeostasis play important roles in atrial fibrillation. Tumor necrosis factor-alpha. (TNF alpha), a proinflammatory cytokine, can induce cardiac arrhythmias. Pulmonary veins (PVs) are critical in initiating paroxysmal atrial fibrillation. This study was designed to investigate whether TNFa may change the calcium handling and arrhythmogenic activity of PV cardiomyocytes. We used whole-cell patch clamp and indo-I fluorimetric ratio technique to investigate the action potentials, ionic currents and intracellular calcium in isolated rabbit single PV cardiomyocytes with and without (control) incubation with TNF alpha (25 ng/ml) for 7-10 h. The expression of sarcoplasmic reticulum ATPase in the control and TNF alpha-treated PV cardiomyocytes was evaluated by confocal micrographs and Western blot. We found that the spontaneous beating rates were similar between the control (n = 45) and TNF alpha-treated (n = 28) PV cardiomyocytes. Compared with the control PV cardiomyocytes, the TNF alpha-treated PV cardiomyocytes had significantly a larger amplitude of the delayed afterdepolarizations (6.0 +/- 1.7 vs. 2.6 +/- 0.8 mV, P