A QUANTITATIVE TEST FOR COPPER USING BICINCHONINIC ACID
A QUANTITATIVE TEST FOR COPPER USING BICINCHONINIC ACID
复制标题
DOI：
10.1006/abio.1995.1194
复制
发表时间：
1995-03-20
影响因子：
2.9
通讯作者：
HARRIS, ED
中科院分区：
文献类型：
--
作者：
BRENNER, AJ;HARRIS, ED
我们描述了一种直接比色法测定血清和生物样品中的铜，使用2，2 '-二喹啉甲酸（BCA），在大多数实验室中的常用试剂。BCA提供了对Cu（I）高度敏感和特异的优点，Cu（I）在BCA的存在下迅速形成强烈的紫色络合物。络合物的最大吸收波长为562和354.5 nm，摩尔吸光系数分别为7.7 × 10 ~（3）和4.6 × 10 ~（4）L mol ~（-1）cm ~（-1）。其他金属离子、pH值和清洁剂的干扰极小，结果与原子吸收分光光度法密切相关。(C)出版社：Academic Press
We describe a direct colorimetric assay for copper in serum and biological samples using 2,2'-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 x 10(3) liter mol(-1) cm(-1) and 4.6 X 10(4) liter mol(-1) cm(-1), respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry. (C) 1995 Academic Press, Inc.