Antigen-antibody interactions: an NMR approach.
Antigen-antibody interactions: an NMR approach.
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DOI：
10.1016/0006-2952(90)90182-k
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发表时间：
1990-07
影响因子：
5.8
通讯作者：
P. Wright;H. Dyson;R. Lerner;L. Riechmann;P. Tsang
中科院分区：
文献类型：
--
作者：
P. Wright;H. Dyson;R. Lerner;L. Riechmann;P. Tsang
随着方法学的最新进展，现在看来NMR可以以前所未有的复杂程度用于获得对抗体结合位点的溶液结构和动力学的新见解，无论是游离的还是与抗原的复合物。在这方面最有前途的是Fv片段（分子量~25 kD），其可以通过基因工程以适合于NMR研究的形式产生。需要同位素标记来进行特定的共振分配。NMR还可以提供免疫原性肽的构象偏好信息，并可用于探测与抗肽抗体Fab片段（分子量约50 kD）结合的肽（适当标记为13 C或15 N）的构象和动力学。
With recent advances in methodology, it now appears that NMR can be used at an unprecedented level of sophistication to obtain new insights into the solution structure and dynamics of the antibody combining site, both free and in its complex with antigen. Most promising in this regard is the Fv fragment (molecular weight ~25 kD) which can be produced by genetic engineering in a form suitable for NMR studies. Isotopic labeling is required to make specific resonance assignments. NMR can also provide information on the conformational preferences of immunogenic peptides and can be used to probe the conformation and dynamics of peptides (appropriately labeled with13C or15N) bound to the Fab fragment (molecular weight ~50 kD) of antipeptide antibodies.