Usefulness of circulating tumor DNA by targeting human papilloma virus-derived sequences as a biomarker in p16-positive oropharyngeal cancer.
Usefulness of circulating tumor DNA by targeting human papilloma virus-derived sequences as a biomarker in p16-positive oropharyngeal cancer.
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DOI：
10.1038/s41598-021-04307-3
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发表时间：
2022-01-12
影响因子：
4.6
通讯作者：
Yamasoba T
中科院分区：
文献类型：
--
作者：
Akashi K;Sakai T;Fukuoka O;Saito Y;Yoshida M;Ando M;Ito T;Murakami Y;Yamasoba T
在头颈部癌症中，早期发现治疗后的复发是重要的。现代治疗药物的发展改善了复发后的预后，然而，还没有建立起用于评估疗效或检测复发的生物标志物。近年来，循环肿瘤DNA(CtDNA)作为一种检测肿瘤的微创、可重复的生物标志物受到了人们的关注。我们通过评估25例p16阳性口咽癌患者，验证了人乳头瘤病毒(HPV)衍生序列的ctDNA作为HPV相关p16阳性口咽癌生物标志物的有效性。在治疗过程中的多个时间点采集每个患者的血液样本，并保存血浆。从血浆中提取ctDNA，用数字聚合酶链式反应进行分析。在25例患者中，14例(56%)检测到HPV来源的ctDNA。在所有患者中，经初步治疗后，样本均为ctDNA阴性。14名患者中有2名癌症复发；复发时检测到HPV来源的ctDNA。我们的结果表明，HPV衍生的ctDNA可以作为预测p16阳性口咽癌复发的一个有前景的生物标志物。
In head and neck cancer, early detection of recurrence after treatment is important. The contemporary development of therapeutic agents have improved the prognosis after recurrence; however, no biomarker has been established for evaluating therapeutic effects or detecting recurrence. Recently, circulating tumor DNA (ctDNA), which comprises DNA derived from tumor cells and exists in the form of cell-free DNA in the blood, has attracted attention as a minimally invasive and repeatable biomarker for detecting cancer. We validated the usefulness of ctDNA of human papilloma virus (HPV)-derived sequences as a biomarker in HPV-related p16-positive oropharyngeal cancer by assessing 25 patients with p16-positive oropharyngeal cancer. Blood samples were collected from each patient at multiple time points during the treatment, and the plasma was preserved. The ctDNA was extracted from the plasma and analyzed using digital polymerase chain reaction. HPV-derived ctDNA was detected in 14 (56%) of the 25 patients. In all the patients, the samples were found to be ctDNA-negative after initial treatment. Cancer recurrence was observed in 2 of the 14 patients; HPV-derived ctDNA was detected at the time of recurrence. Our results indicate that HPV-derived ctDNA can be a prospective biomarker for predicting the recurrence of p16-positive oropharyngeal cancer.
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影响因子：
158.5
作者：
Lynch, TJ;Bell, DW;Haber, DA
通讯作者：
Haber, DA
影响因子：
3.2
作者：
Bharuthram, Avani;Paximadis, Maria;Tiemessen, Caroline T.
通讯作者：
Tiemessen, Caroline T.
影响因子：
11.5
作者：
Chera, Bhishamjit S.;Kumar, Sunil;Gupta, Gaorav P.
通讯作者：
Gupta, Gaorav P.
DOI：
10.1200/jco.2012.45.2011
发表时间：
2014-02-20
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
Diaz LA Jr;Bardelli A
通讯作者：
Bardelli A
影响因子：
17.1
作者：
Wang Y;Springer S;Mulvey CL;Silliman N;Schaefer J;Sausen M;James N;Rettig EM;Guo T;Pickering CR;Bishop JA;Chung CH;Califano JA;Eisele DW;Fakhry C;Gourin CG;Ha PK;Kang H;Kiess A;Koch WM;Myers JN;Quon H;Richmon JD;Sidransky D;Tufano RP;Westra WH;Bettegowda C;Diaz LA Jr;Papadopoulos N;Kinzler KW;Vogelstein B;Agrawal N
通讯作者：
Agrawal N