How do proteins recognize specific RNA sites? New clues from autogenously regulated ribosomal proteins.
How do proteins recognize specific RNA sites? New clues from autogenously regulated ribosomal proteins.
复制标题
蛋白质如何识别特定的RNA位点？
DOI：
10.1016/0968-0004(89)90167-9
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发表时间：
1989
影响因子：
13.8
通讯作者：
Draper,DE
中科院分区：
文献类型：
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作者：
Draper,DE
一些与核糖体RNA特异结合的核糖体蛋白也作为翻译抑制因子，识别它们的编码信使RNA。其中四种蛋白质的信使和核糖体-RNA结合部位现在已经很好地确定了，在大多数情况下，一级和二级结构上的惊人相似之处是显而易见的。这些“共识”结构是蛋白质用来识别特定RNA的特征的有用线索。有一长串已知对基因表达和细胞功能重要的RNA-蛋白质相互作用。其中许多相互作用出现在表达蛋白质所需的重要机制(如剪接体和核糖体)中，而另一些则调节从基因到蛋白质的途径中的步骤。这些相互作用提出了一个问题，即蛋白质如何识别特定的RNA位点。某些类型的RNA结构(螺旋、环、凸起、错配、伪结)是否特别受欢迎作为识别特征？蛋白质是否感知特定的序列、特定的骨架构象或某些组合-
Some ribosomal proteins which bind specifically to ribosomal RNA also act as translational repressors and recognize their encoding messenger RNAs. The messenger-and ribosomal-RNA binding sites for four of these proteins are now well defined, and striking similarities in primary and secondary structure are apparent in most cases. These'consensus' structures are useful clues to the features proteins use to recognize specific RNAs.There is a long list of RNA-protein interactions that are known to be important for gene expression and cell function. Many of these interactions appear in the important machinery needed to express proteins (eg spliceosomes and ribosomes), while others regulate steps in the pathway from gene to protein. These interactions raise the question of how proteins recognize specific RNA sites. Are some kinds of RNA structure~(helices, loops, bulges, mismatches, pseudoknots) particularly favored as recognition features? Do proteins sense specific sequences, particular backbone conformations, or some combina-