BALB- and Harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell.
BALB- and Harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell.
复制标题
新型淋巴祖细胞的BALB和HARVEY-MURINE肉瘤病毒转化。
DOI：
10.1084/jem.156.3.873
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发表时间：
1982-09-01
影响因子：
15.3
通讯作者：
Aaronson, S A
中科院分区：
文献类型：
--
作者：
Pierce, J H;Aaronson, S A
BALB-和Harvey-鼠肉瘤病毒（MSV）包括逆转录病毒家族，其小鼠和大鼠衍生的onc基因密切相关。这些病毒在易感动物中诱发肉瘤和红白血病。体外集落试验，检测转化的淋巴细胞的Abelson-鼠白血病病毒被用来证明BALB-和Harvey-MSV转化一种新的造血细胞在培养和体内。骨髓集落形成依赖于肉瘤病毒，遵循单次打击动力学，并需要在琼脂培养基中存在巯基乙醇。BALB和Harvey-MSV诱导的集落可以在培养物中建立为连续的细胞系，其在体内表现出不受限制的自我更新能力和致白血病性。这些细胞具有原始细胞形态，并且缺乏髓系或红系内成熟细胞的可检测标记物。它们也缺乏可检测的免疫球蛋白μ链或Thy-1抗原，这是通常分别与B和T淋巴谱系的定向细胞相关的标记物。然而，转化体含有非常高水平的末端脱氧核苷酸转移酶（TdT），一种被认为是淋巴分化途径中早期阶段特异性的酶。该表型将这些BALB-和Harvey-MSV转化体与任何先前报道的转化逆转录病毒的造血靶点（包括在相同测定条件下由Abelson-MuLV转化的前B淋巴样细胞）区分开来。这些新发现的淋巴祖细胞转化体可能提供了一个重要的手段，研究淋巴个体发育的早期阶段和TdT在淋巴发育中的可能作用。
BALB- and Harvey-murine sarcoma viruses (MSV) comprise a family of retroviruses whose mouse- and rat-derived onc genes are closely related. These viruses induce sarcomas and erythroleukemias in susceptible animals. An in vitro colony assay that detects transformation of lymphoid cells by Abelson-murine leukemia virus was used to demonstrate that BALB- and Harvey-MSV transform a novel hematopoietic cell both in culture and in vivo. Bone marrow colony formation was sarcoma virus dependent, followed single-hit kinetics, and required the presence of mercaptoethanol in the agar medium. BALB- and Harvey-MSV-induced colonies could be established in culture as continuous cell lines that demonstrated unrestricted self-renewal capacity and leukemogenicity in vivo. The cells had a blast cell morphology and lacked detectable markers of mature cells within the myeloid or erythroid series. They also lacked detectable immunoglobulin mu chain or Thy-1 antigen, markers normally associated with committed cells of the B and T lymphoid lineages, respectively. However, the transformants contained very high levels of terminal deoxynucleotidyl transferase (TdT), an enzyme believed to be specific to early stages within the lymphoid differentiation pathway. This phenotype distinguishes these BALB- and Harvey-MSV transformants from any previously reported hematopoietic targets of transforming retroviruses, including the pre-B lymphoid cell transformed by Abelson-MuLV under identical assay conditions. These newly identified lymphoid progenitor cell transformants may provide an important means of studying early stages of lymphoid ontogeny and the possible role of TdT in lymphoid development.