Nickel chloride administration prevents the growth of oral squamous cell carcinoma.
Nickel chloride administration prevents the growth of oral squamous cell carcinoma.
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DOI：
10.18632/oncotarget.25313
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发表时间：
2018-05-08
期刊：
影响因子：
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通讯作者：
Asano M
中科院分区：
文献类型：
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作者：
Ota H;Shionome T;Suguro H;Saito S;Ueki K;Arai Y;Asano M
研究了NiCl 2对口腔鳞状细胞癌衍生细胞系HSC 3的影响。与1 mM NiCl 2孵育显着降低MMPs的表达在mRNA和蛋白质水平。将高转移亚细胞系HSC 3-M3接种于裸鼠舌面，建立裸鼠原位种植模型。注射1周后，用或不用含ImM NiCl 2的水喂养小鼠2至3周。免疫组织化学检查显示，MMP 9的表达显着减少NiCl 2喂养的小鼠。通过CT图像，在对照小鼠中观察到癌块为半透明区域。在喂食NiCl 2的小鼠中，在半透明区域内观察到许多高度半透明的区域。组织学上，该区域对应于肿瘤块中的坏死区域。实时荧光定量PCR分析显示，减少表达的血管生成因子，如IL-8和VEGF mRNA在NiCl 2喂养的小鼠。为了进一步研究NiCl 2对转移的影响，比较了区域淋巴结中的人β-珠蛋白基因表达。β-珠蛋白基因在NiCl 2喂养的小鼠中完全缺失。此外，通过PCR阵列分析，各种癌症转移相关基因在NiCl 2喂养的小鼠中被抑制。结果表明，NiCl 2可能是一种有前途的新型口腔癌治疗药物。
The effect of NiCl2 on oral squamous cell carcinoma-derived cell line HSC3 was examined. Incubation with 1 mM NiCl2 significantly reduced the expression of MMPs at mRNA and protein levels. The in vivo orthotopic implantation model was established by injecting highly metastatic subcell line HSC3-M3 to nude mouse tongue. After 1 week of injection, mice were fed with or without 1 mM NiCl2-containing water for two to three weeks. Immunohistochamical examination revealed that MMP9 expression was drastically reduced in NiCl2-fed mice. By CT images, cancer mass was observed as a translucent area in control mice. In NiCl2-fed mice, much highly translucent area was observed within the translucent area. Histologically, this area corresponded to the necrotic area in the tumor mass. Real-time PCR analysis revealed the reduced expression of angiogenic factors such as IL-8 and VEGF mRNA in NiCl2-fed mice. To further examine the effect of NiCl2 on metastasis, human β-globin gene expression in regional lymphnodes was compared. The β-globin gene was totaly absent in NiCl2-fed mice. Moreover, various cancer metastasis-related genes were inhibited in NiCl2-fed mice by PCR array analysis. The results indicated that NiCl2 might be a promising new anti-cancer therapeutics for the oral cancer treatment.