DETOXIFICATION OF CEREAL PLANT ALLELOCHEMICALS BY APHIDS - ACTIVITY AND MOLECULAR-WEIGHTS OF GLUTATHIONE-S-TRANSFERASE IN 3 SPECIES OF CEREAL APHIDS
DETOXIFICATION OF CEREAL PLANT ALLELOCHEMICALS BY APHIDS - ACTIVITY AND MOLECULAR-WEIGHTS OF GLUTATHIONE-S-TRANSFERASE IN 3 SPECIES OF CEREAL APHIDS
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DOI：
10.1007/bf02064445
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发表时间：
1994-02-01
影响因子：
2.3
通讯作者：
DIXON, AFG
中科院分区：
文献类型：
--
作者：
LESZCZYNSKI, B;MATOK, M;DIXON, AFG
研究了麦长管蚜、麦长管蚜和禾谷缢管蚜谷胱甘肽S-转移酶的活性和分子量。谷胱甘肽S-转移酶活性的最高水平记录在提取物M。dirhodum和S.麦长管蚜幼虫提取物的活性高于成虫提取物。该酶活性在S. avenae和麦长管蚜R.与易感品种相比，先前饲喂中等抗性小麦品种的Padi。凝胶过滤后SDS-PAGE显示活性组分中有三条蛋白带。第一种分子量为28，500，第二种分子量为27，500，存在于所有三种蚜虫中。第三种蛋白质在三个物种中不同，在S中具有26，000的分子量。燕麦M. dirhodum和R.帕迪。
The activity and molecular weights of glutathione S-transferase were studied in three species of cereal aphid: Metopolophium dirhodum, Sitobion avenae, and Rhopalosiphum padi. The highest level of glutathione S-transferase activity was recorded in extracts from M. dirhodum and the lowest from S. avenae, and extracts of larvae were more active than those from adults. The activity of this enzyme was higher in extracts of S. avenae and R. padi previously fed on a moderately resistant wheat variety than on a susceptible variety. Gel filtration followed by SDS-PAGE revealed three protein bands in the active fractions. The first had a molecular weight of 28,500 and the second of 27,500 and were present in all three species of aphid. The third protein differed in the three species, having a molecular weight of 26,000 in S. avenae, 25,500 in M. dirhodum, and 24,000 in R. padi.