Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung:: a possible correlation with branching morphogenesis
Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung:: a possible correlation with branching morphogenesis
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DOI：
10.1042/cs20030236
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发表时间：
2003-12-01
期刊：
影响因子：
6
通讯作者：
Sasano, H
中科院分区：
文献类型：
--
作者：
Kimura, Y;Suzuki, T;Sasano, H
具有生物活性和强效的雄激素 5α-二氢睾酮 (DHT) 被认为参与人胎肺分支形态发生的发育，但其表达尚未得到检验。因此，我们使用免疫组织化学和逆转录 PCR (RT-PCR) 检查了雄激素受体 (AR) 和 5α 还原酶（1 型和 2 型）的表达，这些酶可催化人胎肺中睾酮转化为 DHT。免疫反应性 AR 主要在妊娠早期胎肺出芽部分的上皮细胞核中检测到。在上皮细胞的细胞质中检测到 5α-还原酶 1 型免疫反应性，而在检查的人胎肺样本中未检测到 5α-还原酶 2 型免疫反应性。 RT-PCR 还证实所有胎肺和上皮细胞系中都存在 AR 和 5a-还原酶。我们目前的研究结果表明，DHT 可能在上皮细胞中发挥重要作用，其中可能包括前体细胞，其中 AR 和 5α 还原酶在人胎肺的分支形态发生过程中表达。
The bioactive and potent androgen 5alpha-dihydrotestosterone (DHT) has been postulated to be involved in the development of branching morphogenesis in the human fetal lung, but its expression has not been examined. We therefore examined the expression of the androgen receptor (AR) and 5alpha-reductases (type 1 and type 2), which catalyse the conversion of testosterone into DHT in the human fetal lung using immunohistochemistry and reverse transcription-PCR (RT-PCR). Immunoreactive AR was detected predominantly in the nuclei of epithelial cells of the budding component of the early gestational fetal lung. 5alpha-Reductase type 1 immunoreactivity was detected in the cytoplasm of epithelial cells, whereas immunoreactivity for 5alpha-reductase type 2 was not detected in the samples of human fetal lung examined. RT-PCR also confirmed the presence of AR and 5a-reductase in all fetal lung and epithelial cell lines. The results of our present study suggest that DHT may play an important role in epithelial cells, which might include precursor cells, in which both AR and 5alpha-reductases are expressed during branching morphogenesis of the human fetal lung.