Transcriptomic Analysis of Seed Germination Under Salt Stress in Two Desert Sister Species (Populus euphratica and P. pruinosa)
Transcriptomic Analysis of Seed Germination Under Salt Stress in Two Desert Sister Species (Populus euphratica and P. pruinosa)
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DOI：
10.3389/fgene.2019.00231
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发表时间：
2019-03
影响因子：
3.7
通讯作者：
Caihua Zhang;Wenchun Luo;Yanda Li;Xu Zhang;Xiaotao Bai;Zhimin Niu;Xiao Zhang;Zhijun Li;Dongshi Wan
中科院分区：
文献类型：
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作者：
Caihua Zhang;Wenchun Luo;Yanda Li;Xu Zhang;Xiaotao Bai;Zhimin Niu;Xiao Zhang;Zhijun Li;Dongshi Wan
作为一种主要的非生物胁迫，土壤盐分限制了种子萌发和植物的生长、发育和生产。种子萌发不仅与幼苗的成活率密切相关，而且与随后的营养生长密切相关。胡杨和粉叶杨是亲缘关系较近的树种，对盐分胁迫表现出明显的适应性。在本研究中，我们对盐胁迫下胡杨和粉刺胡杨种子萌发的三个阶段进行了综合转录组分析。这项研究的二维数据集提供了支持种子萌发和耐盐性的动态生化过程的全面视图。我们的分析在两个物种中发现了12831个种子萌发过程的差异表达基因(Deg)和8071deg的耐盐性基因。此外，我们还确定了每个生长阶段的表达谱和主要途径。对于种子萌发，大量的DEG，包括那些参与能量产生和激素调节途径的DEG，在种子萌发的后期被瞬时和特异地诱导。在两种植物的耐盐性比较中，黄酮类和油菜素类固醇途径显著丰富。更具体地说，在类黄酮途径中，FLS和F3‘5’H表现出显著的差异表达。在油菜素类固醇途径中，DWF4、BR60OX2和ROT3的表达水平显著高于胡杨。我们的结果描述了两个荒漠杨树种子萌发过程中的转录动态，并突出了次生代谢产物参与了对盐胁迫的反应。
As a major abiotic stress, soil salinity limits seed germination and plant growth, development and production. Seed germination is highly related not only to the seedlings survival rate but also subsequent vegetative growth. Populus euphratica and P. pruinosa are closely related species that show a distinguished adaptability to salinity stress. In this study, we performed an integrative transcriptome analyses of three seed germination phases from P. euphratica and P. pruinosa under salt stress. A two-dimensional data set of this study provides a comprehensive view of the dynamic biochemical processes that underpin seed germination and salt tolerance. Our analysis identified 12831 differentially expressed genes (DEGs) for seed germination processes and 8071 DEGs for salt tolerance in the two species. Furthermore, we identified the expression profiles and main pathways in each growth phase. For seed germination, a large number of DEGs, including those involved in energy production and hormonal regulation pathways, were transiently and specifically induced in the late phase. In the comparison of salt tolerance between the two species, the flavonoid and brassinosteroid pathways were significantly enriched. More specifically, in the flavonoid pathway, FLS and F3′5′H exhibited significant differential expression. In the brassinosteroid pathway, the expression levels of DWF4, BR6OX2 and ROT3 were notably higher in P. pruinosa than in P. euphratica. Our results describe transcript dynamics and highlight secondary metabolite pathways involved in the response to salt stress during the seed germination of two desert poplars.