Expression of the 67 kD laminin receptor in human ovarian carcinomas as defined by a monoclonal antibody, MLuC5
Expression of the 67 kD laminin receptor in human ovarian carcinomas as defined by a monoclonal antibody, MLuC5
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DOI：
10.1016/0959-8049(96)00119-0
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发表时间：
1996-08-01
影响因子：
8.4
通讯作者：
Taraboletti, G
中科院分区：
文献类型：
--
作者：
vandenBrule, FA;Castronovo, V;Taraboletti, G
我们实验室先前的免疫组化数据表明，67 kD层粘连蛋白受体（67 LR），一种与癌症相关的高亲和力层粘连蛋白结合蛋白，在卵巢癌细胞中的表达与正常卵巢癌细胞相比上调，并且这种癌细胞中表达的增加可能与患者的预后有关。本研究的目的是验证MLuC5，一种识别67LR的单克隆抗体，作为一种工具，在更大的卵巢癌患者群体中进行未来的免疫组化研究。使用免疫组织化学和MLuC5测定了51例原发性人卵巢癌样本中67LR的表达。在不同程度的卵巢癌细胞簇中检测到67LR。数据分析确定，与完成初级治疗后没有疾病证据的患者相比，来自疾病进展患者的样品中的67LR表达显著增加，并且与边界和1级肿瘤相比，合并的2级和3级肿瘤中的67LR表达显著增加（P < 0.05，卡方检验）。67LR表达与其他临床病理参数之间无显著相关性。这些数据表明，67LR与卵巢肿瘤进展相关。使用该单克隆抗体检测67LR可构成卵巢癌预后确定的一个有趣的参数。版权所有（C）1996 Elsevier Science Ltd
Previous immunohistochemical data from our laboratory have demonstrated that expression of the 67 kD laminin receptor (67LR), a cancer-associated, high-affinity laminin-binding protein, is upregulated in ovarian carcinoma cells compared with normal serosal cells, and that this increased expression in cancer cells could be related to patient outcome. The aim of this study was to validate MLuC5, a monoclonal antibody that recognises the 67LR, as a tool to perform future immunohistochemical studies on larger populations of ovarian carcinoma patients. Expression of the 67LR was determined in 51 primary human ovarian carcinoma samples using immunohistochemistry and MLuC5. The 67LR was detected in ovarian carcinoma cell clusters of variable extent. Analysis of the data determined that 67LR expression was significantly increased in the samples from patients with disease progression, compared with those with no evidence of disease after completion of primary therapy, and in pooled grade 2 and 3 tumours compared to borderline and grade 1 tumours (P < 0.05, chi-squared test). No other significant correlation between 67LR expression and other clinicopathological parameters could be established. These data suggest that the 67LR is correlated to ovarian tumour progression. Detection of the 67LR using this monoclonal antibody could constitute an interesting parameter in prognosis determination of ovarian cancer. Copyright (C) 1996 Elsevier Science Ltd