HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy.
HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy.
复制标题
妊娠早期母体外周血中循环的滋养层细胞中HLA-G的表达。
DOI：
10.1067/mob.2001.112973
复制
发表时间：
2001
影响因子：
9.8
通讯作者：
C. Oudejans
中科院分区：
文献类型：
--
作者：
I. V. van Wijk;S. Griffioen;M. L. Tjoa;M. Mulders;J. V. van Vugt;Y. W. Loke;C. Oudejans
目标
本研究的目的是评估母体外周血中循环滋养层细胞在数字染色体畸变无创产前诊断中的应用。
研究设计
在由密度梯度离心组成的单一富集步骤之后，使用免疫细胞化学鉴定和脱氧核糖核酸荧光原位杂交的组合程序。在脱氧核糖核酸荧光原位杂交中，将特异性 HLA-G 单克隆抗体与 X 和 Y 染色体特异性探针结合使用，以确认男性胎儿中携带 HLA-G 的细胞的胎儿身份。
结果
我们从妊娠 9 周开始在母血中检测到表达 HLA-G 的胎儿滋养层细胞。除了使用 X 和 Y 染色体特异性探针预测胎儿性别外，在患有 21 三体性的妊娠的外周血中也证实了胎儿非整倍性。
结论
尽管胎儿细胞的数量极少，但概念验证得到了证明。早期对胎儿滋养层细胞染色体数量异常进行无创产前筛查是可行的。
OBJECTIVE
The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations.
STUDY DESIGN
A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus.
RESULTS
We detected fetal trophoblast cells expressing HLA-G in maternal blood starting at 9 weeks' gestation. In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21.
CONCLUSION
Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.
影响因子：
15.9
作者：
Zhou, Y;Damsky, CH;Fisher, SJ
通讯作者：
Fisher, SJ