Evidence for divergent projections to the brain noradrenergic system and the spinal parasympathetic system from Barrington's nucleus
Evidence for divergent projections to the brain noradrenergic system and the spinal parasympathetic system from Barrington's nucleus
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DOI：
10.1016/0006-8993(96)00482-9
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发表时间：
1996-09-02
期刊：
影响因子：
2.9
通讯作者：
AstonJones, G
中科院分区：
文献类型：
--
作者：
Valentino, RJ;Chen, S;AstonJones, G
本研究旨在确定位于蓝斑 (LC) 腹内侧并投射到骶副交感核的巴林顿核是否是 LC 传入投射的来源。将顺行示踪剂生物胞素限制性注射到巴林顿核标记的曲张纤维中，该纤维从注射部位延伸到LC。与此一致，将逆向示踪剂、与辣根过氧化物酶偶联的小麦胚芽凝集素与金颗粒 (WGA-Au-HRP) 或荧光素偶联乳胶珠注射到 LC 中，标记了许多（约 10%）Barrington 神经元，这些神经元也通过脊髓中的氟金 (FG) 注射进行了逆行标记。 LC 逆行追踪结合促肾上腺皮质激素释放激素 (CRH) 免疫组织化学显示，巴林顿核中至少三分之一的逆行标记神经元具有 CRH 免疫反应性 (CRH-IR)。最后，在三重标记研究中，一致观察到 CRH-Barrington 神经元从 LC 和脊髓均被逆行标记。这些发现表明巴林顿核是 LC 传入神经和 LC 中 CRH-IR 纤维的来源。此外，结果表明一些巴林顿神经元会分化以同时支配脊髓和 LC。这种发散的神经支配可能有助于共同调节骶副交感神经系统和大脑去甲肾上腺素能系统，从而提供协调骨盆内脏功能与前脑活动的机制。
The present study was designed to determine whether Barrington's nucleus, which lies ventromedial to the locus coeruleus (LC) and projects to the sacral parasympathetic nucleus, is a source of afferent projections to the LC. Restricted injections of the anterograde tracer, biocytin, into Barrington's nucleus labeled varicose fibers that extended from the injection site into the LC. Consistent with this, injections of the retro,grade tracers, wheatgerm agglutinin conjuated to horseradish peroxidase coupled to gold particles (WGA-Au-HRP) or fluorescein-conjugated latex beads, into the LC labeled numerous (approximately 10%) Barrington's neurons that were also retrogradely labeled by Fluoro-Gold (FG) injections in the spinal cord. Retrograde tracing from the LC combined with corticotropin-releasing hormone (CRH) immunohistochemistry revealed that at least one third of the retrogradely labeled neurons in Barrington's nucleus were CRH-immunoreactive (CRH-IR). Finally, in triple labeling studies, CRH-Barrington's neurons were consistently observed that were retrogradely labeled from both the LC and spinal cord. These findings implicate Barrington's nucleus as an LC afferent and a source of CRH-IR fibers in the LC. Additionally, the results suggest that some Barrington's neurons diverge to innervate both the spinal cord and the LC. This divergent innervation may serve to coregulate the sacral parasympathetic nervous system and brain noradrenergic system, thus providing a mechanism for coordinating pelvic visceral functions with forebrain activity.