Quantitative Study of the Effect of Coverage on the Hybridization Efficiency of Surface-Bound DNA Nanostructures
Quantitative Study of the Effect of Coverage on the Hybridization Efficiency of Surface-Bound DNA Nanostructures
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DOI：
10.1021/nl802722k
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发表时间：
2008-12-01
期刊：
影响因子：
10.8
通讯作者：
Casalis, Loredana
中科院分区：
文献类型：
--
作者：
Mirmomtaz, Elham;Castronovo, Matteo;Casalis, Loredana
我们证明，与目前的理解相反，探针分子的密度并不是高密度单链 DNA (ss-DNA) 自组装单层 (SAM) 中缺乏杂交的原因。为此，我们使用纳米接枝技术在金表面惰性硫醇的“地毯基质”SAM 内制造填充良好的 ss-DNA 纳米片。 DNA 表面密度可通过改变“写入”参数（例如尖端速度和扫描线数量）来改变。由于 ss-DNA 的柔韧性比 ds-DNA 高 50 倍，因此杂交会导致过渡到“站立”阶段。因此，在杂交前后准确测量纳米片的高度和可压缩性可以实现可靠、灵敏且无标记的杂交检测。自组装和纳米移植 DNA 单层的并列比较表明，后者虽然比前者更致密，但表现出更高的杂交效率。
We demonstrate that, contrary to current understanding, the density of probe molecules is not responsible for the lack of hybridization in high density single-stranded DNA (ss-DNA) self-assembled monolayers (SAMs). To this end, we use nanografting to fabricate well packed ss-DNA nanopatches within a "carpet matrix" SAM of inert thiols on gold surfaces. The DNA surface density is varied by changing the "writing" parameters, for example, tip speed, and number of scan lines. Since ss-DNA is 50 times more flexible than ds-DNA, hybridization leads to a transition to a "standing up" phase. Therefore, accurate height and compressibility measurements of the nanopatches before and after hybridization allow reliable, sensitive, and label-free detection of hybridization. Side-by-side comparison of self-assembled and nanografted DNA-monolayers shows that the latter, while denser than the former, display higher hybridization efficiencies.