The neuropeptide complement of the marine annelid Platynereis dumerilii.
The neuropeptide complement of the marine annelid Platynereis dumerilii.
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DOI：
10.1186/1471-2164-14-906
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发表时间：
2013-12-20
期刊：
影响因子：
4.4
通讯作者：
Jékely G
中科院分区：
文献类型：
--
作者：
Conzelmann M;Williams EA;Krug K;Franz-Wachtel M;Macek B;Jékely G
海洋环节动物Platynereis dumerilii正在成为进化发育生物学和神经生物学的一个强大的实验模型。最近的研究表明，存在保守的神经肽能信号，包括催产素/neurophysin，肌抑制肽和阿片肽系统。尽管有这些进展，但尚未报道全面的肽组资源。本工作描述了扁虫属的神经肽组。我们建立了一个大型转录组资源，包括阶段特异性下一代测序数据集和77，419个表达序列标签。使用这些信息和生物信息学搜索和质谱分析的组合，我们增加了已知的原神经肽（PNP）的补充，以98。基于与后生动物pNPs的序列同源性，将Platynereis pNPs分为古真后生动物、两侧体动物、原口动物、Lophotrochozoan和环节动物家族，并且仅在Platynereis中发现pNPs。相比，唯一的其他lophotrochozoan与大规模的pNP资源的Planarian Schmidtea meditriquea，Platynereis有一个非常完整的保守的pNPs的补充，与53 pNPs属于古老的真后生动物或bilaterian家庭。我们全面的搜索策略，结合序列保守性分析，也使我们能够定义几个新的lophotrochozoan和环节动物pNP家庭。阶段特异性转录组数据集也使我们能够在整个Platynereis生命周期中绘制pNP表达的变化。大剧目的保守pNPs在Platynereis突出了环节动物的有用性比较神经内分泌学。这项工作建立了一个参考数据集的比较肽在lophotrochozoans，并提供了基础，为今后的研究，扁水母肽能信号。
The marine annelid Platynereis dumerilii is emerging as a powerful lophotrochozoan experimental model for evolutionary developmental biology (evo-devo) and neurobiology. Recent studies revealed the presence of conserved neuropeptidergic signaling in Platynereis, including vasotocin/neurophysin, myoinhibitory peptide and opioid peptidergic systems. Despite these advances, comprehensive peptidome resources have yet to be reported. The present work describes the neuropeptidome of Platynereis. We established a large transcriptome resource, consisting of stage-specific next-generation sequencing datasets and 77,419 expressed sequence tags. Using this information and a combination of bioinformatic searches and mass spectrometry analyses, we increased the known proneuropeptide (pNP) complement of Platynereis to 98. Based on sequence homology to metazoan pNPs, Platynereis pNPs were grouped into ancient eumetazoan, bilaterian, protostome, lophotrochozoan, and annelid families, and pNPs only found in Platynereis. Compared to the planarian Schmidtea mediterranea, the only other lophotrochozoan with a large-scale pNP resource, Platynereis has a remarkably full complement of conserved pNPs, with 53 pNPs belonging to ancient eumetazoan or bilaterian families. Our comprehensive search strategy, combined with analyses of sequence conservation, also allowed us to define several novel lophotrochozoan and annelid pNP families. The stage-specific transcriptome datasets also allowed us to map changes in pNP expression throughout the Platynereis life cycle. The large repertoire of conserved pNPs in Platynereis highlights the usefulness of annelids in comparative neuroendocrinology. This work establishes a reference dataset for comparative peptidomics in lophotrochozoans and provides the basis for future studies of Platynereis peptidergic signaling.