A novel and simple ORAC methodology based on the interaction of Pyrogallol Red with peroxyl radicals
A novel and simple ORAC methodology based on the interaction of Pyrogallol Red with peroxyl radicals
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DOI：
10.1080/10715760500481233
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发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Lissi, E.
中科院分区：
文献类型：
--
作者：
Lopez-Alarcon, C.;Lissi, E.
氧自由基吸收容量（ORAC）指数经常用来表征纯化合物及其复杂混合物的自由基捕获能力。使用藻红蛋白、荧光素（FL）或c-藻蓝蛋白作为靶标获得的ORAC值的缺点使得可以得出结论，对于非常反应性的化合物，它们与化学计量因子的相关性比与测试化合物的反应性的相关性大得多。在本文中，我们提出了一种简单的方法，基于漂白邻苯三酚红（PGR）的吸光度，提供ORAC指数，几乎完全由测试化合物的反应性确定。这种差异是由于PCR的高反应性和实验中使用的该化合物的高浓度。
Oxygen radicals absorbance capacities (ORAC) indexes are frequently employed to characterize the radical trapping capacity of pure compounds and their complex mixtures. A drawback of ORAC values obtained using phycoerythrin, fluorescein (FL) or c-phycocyanin as targets, makes it possible to conclude that for very reactive compounds they are much more related to stoichiometric factors than to the reactivity of the tested compound. In the present paper, we propose a simple methodology, based on the bleaching of Pyrogallol Red (PGR) absorbance that provides ORAC indexes that are almost exclusively determined by the reactivity of the tested compounds. This difference is due to the high reactivity of PGR and the high concentrations of this compound employed in the experiments.